Detailed information of TA system TA090303

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 5115142..5115554
• Replicon: chromosome
• Accession: NZ_CP025401
• Organism: Escherichia coli strain MS8345

Toxin (Protein)

• Gene name: symE
• Uniprot ID: A0A8E0IWC4
• Locus tag: MS8345_RS26810
• Protein ID: WP_001513534.1
• Coordinates: 5115142..5115483 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	5115478..5115554 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS8345_RS26790	5110211..5111491	-	1281	WP_001338077.1	DUF445 domain-containing protein	-
MS8345_RS26795	5111676..5112596	+	921	WP_001513532.1	Rpn family recombination-promoting nuclease/putative transposase	-
MS8345_RS26800	5112824..5112904	+	81	WP_020233658.1	hypothetical protein	-
MS8345_RS26805	5112939..5115095	+	2157	WP_001513533.1	DUF262 domain-containing protein	-
MS8345_RS26810	5115142..5115483	-	342	WP_001513534.1	endoribonuclease SymE	Toxin
-	5115478..5115554	+	77	NuclAT_6	-	Antitoxin
-	5115478..5115554	+	77	NuclAT_6	-	Antitoxin
-	5115478..5115554	+	77	NuclAT_6	-	Antitoxin
-	5115478..5115554	+	77	NuclAT_6	-	Antitoxin
-	5115478..5115554	+	77	NuclAT_7	-	Antitoxin
-	5115478..5115554	+	77	NuclAT_7	-	Antitoxin
-	5115478..5115554	+	77	NuclAT_7	-	Antitoxin
-	5115478..5115554	+	77	NuclAT_7	-	Antitoxin
MS8345_RS26815	5115704..5117473	-	1770	WP_001513535.1	restriction endonuclease subunit S	-
MS8345_RS26820	5117473..5118942	-	1470	WP_001387312.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12247.03 Da
• Isoelectric Point: 7.8545

>T90303 WP_001513534.1 NZ_CP025401:c5115483-5115142 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASHYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T90303 NZ_CP025401:c5115483-5115142 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCACTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACTGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT90303 NZ_CP025401:5115478-5115554 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Antitoxin