Detailed information of TA system TA090298

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4442037..4442639
• Replicon: chromosome
• Accession: NZ_CP025401
• Organism: Escherichia coli strain MS8345

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: MS8345_RS23250
• Protein ID: WP_000897302.1
• Coordinates: 4442037..4442348 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MS8345_RS23255
• Protein ID: WP_000356397.1
• Coordinates: 4442349..4442639 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS8345_RS23225	4437951..4438550	+	600	WP_001314324.1	glucose-1-phosphatase	-
MS8345_RS23230	4438544..4439416	+	873	WP_000920762.1	virulence factor BrkB family protein	-
MS8345_RS23235	4439413..4439850	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
MS8345_RS23240	4439895..4440836	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
MS8345_RS23245	4440900..4441808	-	909	WP_001331553.1	alpha/beta hydrolase	-
MS8345_RS23250	4442037..4442348	+	312	WP_000897302.1	hypothetical protein	Toxin
MS8345_RS23255	4442349..4442639	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
MS8345_RS23265	4442998..4443276	+	279	WP_001296612.1	hypothetical protein	-
MS8345_RS23275	4443672..4443890	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
MS8345_RS23280	4444106..4445035	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
MS8345_RS23285	4445032..4445667	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
MS8345_RS23290	4445664..4446566	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T90298 WP_000897302.1 NZ_CP025401:4442037-4442348 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T90298 NZ_CP025401:4442037-4442348 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT90298 WP_000356397.1 NZ_CP025401:4442349-4442639 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT90298 NZ_CP025401:4442349-4442639 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416