Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2168278..2168780 Replicon chromosome
Accession NZ_CP025401
Organism Escherichia coli strain MS8345

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag MS8345_RS11560 Protein ID WP_000767829.1
Coordinates 2168278..2168532 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag MS8345_RS11565 Protein ID WP_001259255.1
Coordinates 2168529..2168780 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS8345_RS11530 2163293..2163520 - 228 WP_083574903.1 sulfurtransferase TusA family protein -
MS8345_RS11535 2163534..2164592 - 1059 WP_000492340.1 transport protein YeeE -
MS8345_RS11540 2164771..2166129 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
MS8345_RS11545 2166119..2166181 - 63 WP_010723108.1 membrane protein YoeI -
MS8345_RS11550 2166396..2167325 - 930 WP_000803351.1 LysR family transcriptional regulator -
MS8345_RS11555 2167371..2168195 - 825 WP_000754737.1 SDR family oxidoreductase -
MS8345_RS11560 2168278..2168532 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
MS8345_RS11565 2168529..2168780 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
MS8345_RS11570 2169063..2169113 + 51 WP_071593552.1 his operon leader peptide -
MS8345_RS11580 2169259..2170158 + 900 WP_000131789.1 ATP phosphoribosyltransferase -
MS8345_RS11585 2170164..2171468 + 1305 WP_001513845.1 histidinol dehydrogenase -
MS8345_RS11590 2171465..2172535 + 1071 WP_016239284.1 histidinol-phosphate transaminase -
MS8345_RS11595 2172535..2173602 + 1068 WP_001513846.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T90286 WP_000767829.1 NZ_CP025401:c2168532-2168278 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T90286 NZ_CP025401:c2168532-2168278 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT90286 WP_001259255.1 NZ_CP025401:c2168780-2168529 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT90286 NZ_CP025401:c2168780-2168529 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References