90286

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2168278..2168780 Replicon chromosome
Accession NZ_CP025401
Organism Escherichia coli strain MS8345

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag MS8345_RS11560 Protein ID WP_000767829.1
Coordinates 2168278..2168532 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag MS8345_RS11565 Protein ID WP_001259255.1
Coordinates 2168529..2168780 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS8345_RS11530 2163293..2163520 - 228 WP_083574903.1 sulfurtransferase TusA family protein -
MS8345_RS11535 2163534..2164592 - 1059 WP_000492340.1 transport protein YeeE -
MS8345_RS11540 2164771..2166129 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
MS8345_RS11545 2166119..2166181 - 63 WP_010723108.1 membrane protein YoeI -
MS8345_RS11550 2166396..2167325 - 930 WP_000803351.1 LysR family transcriptional regulator -
MS8345_RS11555 2167371..2168195 - 825 WP_000754737.1 SDR family oxidoreductase -
MS8345_RS11560 2168278..2168532 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
MS8345_RS11565 2168529..2168780 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
MS8345_RS11570 2169063..2169113 + 51 WP_071593552.1 his operon leader peptide -
MS8345_RS11580 2169259..2170158 + 900 WP_000131789.1 ATP phosphoribosyltransferase -
MS8345_RS11585 2170164..2171468 + 1305 WP_001513845.1 histidinol dehydrogenase -
MS8345_RS11590 2171465..2172535 + 1071 WP_016239284.1 histidinol-phosphate transaminase -
MS8345_RS11595 2172535..2173602 + 1068 WP_001513846.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T90286 WP_000767829.1 NZ_CP025401:c2168532-2168278 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T90286 NZ_CP025401:c2168532-2168278 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT90286 WP_001259255.1 NZ_CP025401:c2168780-2168529 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT90286 NZ_CP025401:c2168780-2168529 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References