Detailed information of TA system TA090271

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 284010..284704
• Replicon: chromosome
• Accession: NZ_CP025401
• Organism: Escherichia coli strain MS8345

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: MS8345_RS01365
• Protein ID: WP_001263500.1
• Coordinates: 284306..284704 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: MS8345_RS01360
• Protein ID: WP_000554758.1
• Coordinates: 284010..284303 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS8345_RS01340	279546..280043	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
MS8345_RS29610	280126..280284	-	159	WP_014639450.1	hypothetical protein	-
MS8345_RS01345	280363..282075	-	1713	Protein_253	flagellar biosynthesis protein FlhA	-
MS8345_RS01350	282047..282832	+	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
MS8345_RS01355	282903..283958	+	1056	WP_001226168.1	DNA polymerase IV	-
MS8345_RS01360	284010..284303	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
MS8345_RS01365	284306..284704	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
MS8345_RS01370	284714..285166	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
MS8345_RS01375	285356..286495	+	1140	WP_000521577.1	RNA ligase RtcB family protein	-
MS8345_RS01380	286492..287106	+	615	WP_000602123.1	peptide chain release factor H	-
MS8345_RS01385	287163..288620	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
MS8345_RS01390	288881..289339	+	459	WP_001291988.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	264486..285166	20680

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T90271 WP_001263500.1 NZ_CP025401:284306-284704 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T90271 NZ_CP025401:284306-284704 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT90271 WP_000554758.1 NZ_CP025401:284010-284303 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT90271 NZ_CP025401:284010-284303 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3