Detailed information of TA system TA090248

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): holin-SprF2/-
• Location: 370477..370995
• Replicon: chromosome
• Accession: NZ_CP025395
• Organism: Staphylococcus aureus O46

Toxin (Protein)

• Gene name: holin
• Uniprot ID: -
• Locus tag: SAO46_RS01735
• Protein ID: WP_000448764.1
• Coordinates: 370693..370995 (+)
• Length: 101 a.a.

Antitoxin (RNA)

Gene name	SprF2
Locus tag	-
Coordinates	370477..370613 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAO46_RS01695	365571..365861	+	291	WP_000179860.1	hypothetical protein	-
SAO46_RS01700	365877..367787	+	1911	WP_000429563.1	hypothetical protein	-
SAO46_RS01705	367787..369253	+	1467	WP_000067157.1	BppU family phage baseplate upper protein	-
SAO46_RS01710	369253..369642	+	390	WP_001166604.1	DUF2977 domain-containing protein	-
SAO46_RS01715	369635..369799	+	165	WP_000916020.1	XkdX family protein	-
SAO46_RS01720	369845..370144	+	300	WP_000466773.1	DUF2951 family protein	-
SAO46_RS14350	370296..370485	+	190	Protein_327	putative holin-like toxin	-
-	370477..370613	-	137	NuclAT_0	-	Antitoxin
-	370477..370613	-	137	NuclAT_0	-	Antitoxin
-	370477..370613	-	137	NuclAT_0	-	Antitoxin
-	370477..370613	-	137	NuclAT_0	-	Antitoxin
SAO46_RS01730	370532..370642	-	111	WP_070003492.1	hypothetical protein	-
SAO46_RS01735	370693..370995	+	303	WP_000448764.1	phage holin	Toxin
SAO46_RS01740	371007..372461	+	1455	WP_000930277.1	N-acetylmuramoyl-L-alanine amidase	-
SAO46_RS01750	373974..374471	+	498	WP_001803960.1	hypothetical protein	-
SAO46_RS01760	375031..375858	-	828	WP_000136010.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	geh	319286..372461	53175

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Holin (3-68)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11230.99 Da
• Isoelectric Point: 9.6587

>T90248 WP_000448764.1 NZ_CP025395:370693-370995 [Staphylococcus aureus O46]
METKVITRYIVLILALVNQFLANKGISPIPVDEESISSIILTVIALYTAYKDNPTSQEGRWANQKLKKYKAENKYRKATG
QAPIKEVMTPTNMNDTNDLG

Nucleotide

• Length: 303 bp

>T90248 NZ_CP025395:370693-370995 [Staphylococcus aureus O46]
ATGGAAACAAAAGTAATAACAAGATATATTGTATTAATTTTAGCGTTAGTCAATCAGTTTTTAGCGAACAAAGGTATAAG
CCCGATACCAGTAGATGAAGAGAGTATTTCATCGATTATCTTAACAGTCATTGCTTTATACACCGCTTATAAAGACAATC
CAACATCACAAGAAGGACGCTGGGCGAATCAAAAACTAAAGAAATATAAAGCTGAAAATAAGTATAGAAAAGCAACAGGA
CAAGCGCCAATTAAAGAAGTAATGACACCTACAAATATGAATGACACAAATGATTTAGGGTAG

Antitoxin

Nucleotide

• Length: 137 bp

>AT90248 NZ_CP025395:c370613-370477 [Staphylococcus aureus O46]
ATATATAGAAAAAGGGCAACATGCGGAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGACCTCTGTAGTTAAAT
GAATTTATATAATCCTCTAACCATCGTACTCGTCAAAGTTAGCGATGGTTATTTTTT

Structures

Antitoxin