Detailed information of TA system TA090148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1785999..1786589
• Replicon: chromosome
• Accession: NZ_CP025334
• Organism: Brevibacterium aurantiacum strain SMQ-1420

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A2A3ZLA3
• Locus tag: CXR27_RS08090
• Protein ID: WP_009883719.1
• Coordinates: 1785999..1786280 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CXR27_RS08095
• Protein ID: WP_009883720.1
• Coordinates: 1786290..1786589 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXR27_RS08055	1781150..1781870	+	721	Protein_1589	TetR/AcrR family transcriptional regulator	-
CXR27_RS08065	1782268..1782954	-	687	WP_009883715.1	haloacid dehalogenase type II	-
CXR27_RS08070	1783134..1783724	-	591	WP_009883716.1	GNAT family N-acetyltransferase	-
CXR27_RS08075	1783721..1784326	-	606	WP_096158601.1	TetR family transcriptional regulator	-
CXR27_RS08080	1784497..1784862	+	366	WP_069599925.1	alkylphosphonate utilization protein	-
CXR27_RS08090	1785999..1786280	+	282	WP_009883719.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CXR27_RS08095	1786290..1786589	+	300	WP_009883720.1	HigA family addiction module antidote protein	Antitoxin
CXR27_RS08100	1786623..1787813	+	1191	WP_127362044.1	PD-(D/E)XK nuclease family protein	-
CXR27_RS08105	1787890..1788930	-	1041	WP_035278961.1	methionine synthase	-
CXR27_RS08110	1788948..1789940	-	993	WP_069599929.1	DUF1852 domain-containing protein	-
CXR27_RS08115	1790066..1791007	+	942	WP_029417202.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-69)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10928.30 Da
• Isoelectric Point: 10.7346

>T90148 WP_009883719.1 NZ_CP025334:1785999-1786280 [Brevibacterium aurantiacum]
VIRSFADRDTERVWRREPARRLDPRIHKTANRKLHQLDAAVSLDSMRVPPGNRLESLRGDRAGQHSIRINDQWRICFRWT
GAGAEEVEIVDYH

Nucleotide

• Length: 282 bp

>T90148 NZ_CP025334:1785999-1786280 [Brevibacterium aurantiacum]
GTGATCAGATCGTTTGCCGACCGCGACACCGAGCGTGTGTGGCGTCGTGAACCGGCGAGGCGACTTGATCCTCGTATTCA
CAAGACTGCGAATCGGAAGTTGCATCAGCTGGATGCAGCGGTGTCGCTCGATTCCATGCGTGTTCCGCCCGGTAATCGTC
TGGAGTCACTCAGAGGTGATCGGGCTGGTCAGCATAGCATTCGCATCAACGATCAGTGGCGGATCTGCTTTAGGTGGACC
GGTGCCGGTGCAGAAGAAGTCGAGATCGTGGACTATCACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11132.84 Da
• Isoelectric Point: 5.7011

>AT90148 WP_009883720.1 NZ_CP025334:1786290-1786589 [Brevibacterium aurantiacum]
MSEKLYPPIHPGEVLMEDFIEGFGITQNKLAVSIGVPPRRINEIVHGKRAITADTALRLGRYFGVEPQFWLNLQSRYDLE
LAEERVSDQVAAITPLKVA

Nucleotide

• Length: 300 bp

>AT90148 NZ_CP025334:1786290-1786589 [Brevibacterium aurantiacum]
ATGTCCGAGAAGCTTTACCCGCCGATCCATCCGGGGGAAGTCCTCATGGAGGATTTCATCGAGGGGTTCGGTATCACGCA
GAATAAGCTCGCTGTGTCGATCGGTGTGCCACCTCGGCGTATCAATGAAATAGTGCACGGAAAGCGTGCCATCACTGCCG
ACACAGCTTTGCGTTTGGGGCGGTATTTCGGTGTGGAGCCGCAATTCTGGCTTAACCTGCAGAGTCGTTATGATCTGGAG
CTTGCAGAGGAACGCGTGTCCGACCAGGTCGCGGCAATCACGCCGCTTAAGGTTGCGTAA

Structures

Toxin

• AlphaFold DB: A0A2A3ZLA3