Detailed information of TA system TA090147

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1658399..1658989
• Replicon: chromosome
• Accession: NZ_CP025333
• Organism: Brevibacterium aurantiacum strain SMQ-1419

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A2A3ZLA3
• Locus tag: CXR25_RS07610
• Protein ID: WP_009883719.1
• Coordinates: 1658399..1658680 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CXR25_RS07615
• Protein ID: WP_009883720.1
• Coordinates: 1658690..1658989 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXR25_RS07585	1653549..1654655	+	1107	WP_101583139.1	DNA alkylation repair protein	-
CXR25_RS07590	1654643..1655050	-	408	WP_125240482.1	VOC family protein	-
CXR25_RS07595	1655584..1656783	+	1200	WP_125240483.1	MFS transporter	-
CXR25_RS07600	1656897..1657262	+	366	WP_069599925.1	alkylphosphonate utilization protein	-
CXR25_RS07610	1658399..1658680	+	282	WP_009883719.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CXR25_RS07615	1658690..1658989	+	300	WP_009883720.1	HigA family addiction module antidote protein	Antitoxin
CXR25_RS07620	1659044..1660213	+	1170	WP_101583634.1	PD-(D/E)XK nuclease family protein	-
CXR25_RS07625	1660290..1661330	-	1041	WP_101583632.1	methionine synthase	-
CXR25_RS07630	1661348..1662340	-	993	WP_101583623.1	DUF1852 domain-containing protein	-
CXR25_RS07635	1662466..1663407	+	942	WP_029417202.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1651290..1660267	8977

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-69)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10928.30 Da
• Isoelectric Point: 10.7346

>T90147 WP_009883719.1 NZ_CP025333:1658399-1658680 [Brevibacterium aurantiacum]
VIRSFADRDTERVWRREPARRLDPRIHKTANRKLHQLDAAVSLDSMRVPPGNRLESLRGDRAGQHSIRINDQWRICFRWT
GAGAEEVEIVDYH

Nucleotide

• Length: 282 bp

>T90147 NZ_CP025333:1658399-1658680 [Brevibacterium aurantiacum]
GTGATCAGATCGTTTGCCGACCGCGACACCGAGCGTGTGTGGCGTCGTGAACCGGCGAGGCGACTTGATCCTCGTATTCA
CAAGACTGCGAATCGGAAGTTGCATCAGCTGGATGCAGCGGTGTCGCTCGATTCCATGCGTGTTCCGCCCGGTAATCGTC
TGGAGTCACTCAGAGGTGATCGGGCTGGTCAGCATAGCATTCGCATCAACGATCAGTGGCGGATCTGCTTTAGGTGGACC
GGTGCCGGTGCAGAAGAAGTCGAGATCGTGGACTATCACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11132.84 Da
• Isoelectric Point: 5.7011

>AT90147 WP_009883720.1 NZ_CP025333:1658690-1658989 [Brevibacterium aurantiacum]
MSEKLYPPIHPGEVLMEDFIEGFGITQNKLAVSIGVPPRRINEIVHGKRAITADTALRLGRYFGVEPQFWLNLQSRYDLE
LAEERVSDQVAAITPLKVA

Nucleotide

• Length: 300 bp

>AT90147 NZ_CP025333:1658690-1658989 [Brevibacterium aurantiacum]
ATGTCCGAGAAGCTTTACCCGCCGATCCATCCGGGGGAAGTCCTCATGGAGGATTTCATCGAGGGGTTCGGTATCACGCA
GAATAAGCTCGCTGTGTCGATCGGTGTGCCACCTCGGCGTATCAATGAAATAGTGCACGGAAAGCGTGCCATCACTGCCG
ACACAGCTTTGCGTTTGGGGCGGTATTTCGGTGTGGAGCCGCAATTCTGGCTTAACCTGCAGAGTCGTTATGATCTGGAG
CTTGCAGAGGAACGCGTGTCCGACCAGGTCGCGGCAATCACGCCGCTTAAGGTTGCGTAA

Structures

Toxin

• AlphaFold DB: A0A2A3ZLA3