Detailed information of TA system TA090146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1617698..1618288
• Replicon: chromosome
• Accession: NZ_CP025332
• Organism: Brevibacterium aurantiacum strain SMQ-1421

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A2A3ZLA3
• Locus tag: CXR26_RS07465
• Protein ID: WP_009883719.1
• Coordinates: 1617698..1617979 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CXR26_RS07470
• Protein ID: WP_009883720.1
• Coordinates: 1617989..1618288 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXR26_RS07440	1613109..1613699	-	591	WP_096146698.1	GNAT family N-acetyltransferase	-
CXR26_RS07445	1613696..1614301	-	606	WP_096146697.1	TetR/AcrR family transcriptional regulator	-
CXR26_RS07450	1614883..1616082	+	1200	WP_096146696.1	MFS transporter	-
CXR26_RS07455	1616196..1616561	+	366	Protein_1468	alkylphosphonate utilization protein	-
CXR26_RS07465	1617698..1617979	+	282	WP_009883719.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CXR26_RS07470	1617989..1618288	+	300	WP_009883720.1	HigA family addiction module antidote protein	Antitoxin
CXR26_RS07475	1618322..1619512	+	1191	WP_096146801.1	PD-(D/E)XK nuclease family protein	-
CXR26_RS07480	1619589..1620629	-	1041	WP_035278961.1	methionine synthase	-
CXR26_RS07485	1620647..1621639	-	993	WP_069599929.1	DUF1852 domain-containing protein	-
CXR26_RS07490	1621765..1622706	+	942	WP_029417202.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-69)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10928.30 Da
• Isoelectric Point: 10.7346

>T90146 WP_009883719.1 NZ_CP025332:1617698-1617979 [Brevibacterium aurantiacum]
VIRSFADRDTERVWRREPARRLDPRIHKTANRKLHQLDAAVSLDSMRVPPGNRLESLRGDRAGQHSIRINDQWRICFRWT
GAGAEEVEIVDYH

Nucleotide

• Length: 282 bp

>T90146 NZ_CP025332:1617698-1617979 [Brevibacterium aurantiacum]
GTGATCAGATCGTTTGCCGACCGCGACACCGAGCGTGTGTGGCGTCGTGAACCGGCGAGGCGACTTGATCCTCGTATTCA
CAAGACTGCGAATCGGAAGTTGCATCAGCTGGATGCAGCGGTGTCGCTCGATTCCATGCGTGTTCCGCCCGGTAATCGTC
TGGAGTCACTCAGAGGTGATCGGGCTGGTCAGCATAGCATTCGCATCAACGATCAGTGGCGGATCTGCTTTAGGTGGACC
GGTGCCGGTGCAGAAGAAGTCGAGATCGTGGACTATCACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11132.84 Da
• Isoelectric Point: 5.7011

>AT90146 WP_009883720.1 NZ_CP025332:1617989-1618288 [Brevibacterium aurantiacum]
MSEKLYPPIHPGEVLMEDFIEGFGITQNKLAVSIGVPPRRINEIVHGKRAITADTALRLGRYFGVEPQFWLNLQSRYDLE
LAEERVSDQVAAITPLKVA

Nucleotide

• Length: 300 bp

>AT90146 NZ_CP025332:1617989-1618288 [Brevibacterium aurantiacum]
ATGTCCGAGAAGCTTTACCCGCCGATCCATCCGGGGGAAGTCCTCATGGAGGATTTCATCGAGGGGTTCGGTATCACGCA
GAATAAGCTCGCTGTGTCGATCGGTGTGCCACCTCGGCGTATCAATGAAATAGTGCACGGAAAGCGTGCCATCACTGCCG
ACACAGCTTTGCGTTTGGGGCGGTATTTCGGTGTGGAGCCGCAATTCTGGCTTAACCTGCAGAGTCGTTATGATCTGGAG
CTTGCAGAGGAACGCGTGTCCGACCAGGTCGCGGCAATCACGCCGCTTAAGGTTGCGTAA

Structures

Toxin

• AlphaFold DB: A0A2A3ZLA3