Detailed information of TA system TA090145

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1709164..1709754
• Replicon: chromosome
• Accession: NZ_CP025331
• Organism: Brevibacterium aurantiacum strain SMQ-1418

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A2A3ZLA3
• Locus tag: CXR24_RS07755
• Protein ID: WP_009883719.1
• Coordinates: 1709164..1709445 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CXR24_RS07760
• Protein ID: WP_009883720.1
• Coordinates: 1709455..1709754 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXR24_RS07735	1705087..1705452	+	366	Protein_1526	alkylphosphonate utilization protein	-
CXR24_RS07745	1706096..1707646	+	1551	WP_009884100.1	IS21 family transposase	-
CXR24_RS07750	1707685..1708389	+	705	WP_205677020.1	ATP-binding protein	-
CXR24_RS07755	1709164..1709445	+	282	WP_009883719.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CXR24_RS07760	1709455..1709754	+	300	WP_009883720.1	HigA family addiction module antidote protein	Antitoxin
CXR24_RS07765	1709788..1710978	+	1191	WP_125178076.1	PD-(D/E)XK nuclease family protein	-
CXR24_RS07770	1711055..1712095	-	1041	WP_035278961.1	methionine synthase	-
CXR24_RS07775	1712113..1713105	-	993	WP_069599929.1	DUF1852 domain-containing protein	-
CXR24_RS07780	1713231..1714172	+	942	WP_125178077.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1707643..1708389	746

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-69)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10928.30 Da
• Isoelectric Point: 10.7346

>T90145 WP_009883719.1 NZ_CP025331:1709164-1709445 [Brevibacterium aurantiacum]
VIRSFADRDTERVWRREPARRLDPRIHKTANRKLHQLDAAVSLDSMRVPPGNRLESLRGDRAGQHSIRINDQWRICFRWT
GAGAEEVEIVDYH

Nucleotide

• Length: 282 bp

>T90145 NZ_CP025331:1709164-1709445 [Brevibacterium aurantiacum]
GTGATCAGATCGTTTGCCGACCGCGACACCGAGCGTGTGTGGCGTCGTGAACCGGCGAGGCGACTTGATCCTCGTATTCA
CAAGACTGCGAATCGGAAGTTGCATCAGCTGGATGCAGCGGTGTCGCTCGATTCCATGCGTGTTCCGCCCGGTAATCGTC
TGGAGTCACTCAGAGGTGATCGGGCTGGTCAGCATAGCATTCGCATCAACGATCAGTGGCGGATCTGCTTTAGGTGGACC
GGTGCCGGTGCAGAAGAAGTCGAGATCGTGGACTATCACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11132.84 Da
• Isoelectric Point: 5.7011

>AT90145 WP_009883720.1 NZ_CP025331:1709455-1709754 [Brevibacterium aurantiacum]
MSEKLYPPIHPGEVLMEDFIEGFGITQNKLAVSIGVPPRRINEIVHGKRAITADTALRLGRYFGVEPQFWLNLQSRYDLE
LAEERVSDQVAAITPLKVA

Nucleotide

• Length: 300 bp

>AT90145 NZ_CP025331:1709455-1709754 [Brevibacterium aurantiacum]
ATGTCCGAGAAGCTTTACCCGCCGATCCATCCGGGGGAAGTCCTCATGGAGGATTTCATCGAGGGGTTCGGTATCACGCA
GAATAAGCTCGCTGTGTCGATCGGTGTGCCACCTCGGCGTATCAATGAAATAGTGCACGGAAAGCGTGCCATCACTGCCG
ACACAGCTTTGCGTTTGGGGCGGTATTTCGGTGTGGAGCCGCAATTCTGGCTTAACCTGCAGAGTCGTTATGATCTGGAG
CTTGCAGAGGAACGCGTGTCCGACCAGGTCGCGGCAATCACGCCGCTTAAGGTTGCGTAA

Structures

Toxin

• AlphaFold DB: A0A2A3ZLA3