Detailed information of TA system TA090144

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1829557..1830147
• Replicon: chromosome
• Accession: NZ_CP025330
• Organism: Brevibacterium aurantiacum strain SMQ-1417

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A2A3ZLA3
• Locus tag: CXR23_RS08420
• Protein ID: WP_009883719.1
• Coordinates: 1829557..1829838 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CXR23_RS08425
• Protein ID: WP_009883720.1
• Coordinates: 1829848..1830147 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXR23_RS08390	1824804..1826079	-	1276	Protein_1654	IS1380 family transposase	-
CXR23_RS08395	1826052..1826348	+	297	WP_096162045.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CXR23_RS08400	1826335..1826637	+	303	WP_096162044.1	HigA family addiction module antidote protein	-
CXR23_RS20430	1826944..1827426	+	483	WP_205676697.1	2-phosphosulfolactate phosphatase	-
CXR23_RS08410	1827542..1827931	-	390	WP_096162042.1	VOC family protein	-
CXR23_RS08415	1828055..1828420	+	366	WP_069599925.1	alkylphosphonate utilization protein	-
CXR23_RS08420	1829557..1829838	+	282	WP_009883719.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CXR23_RS08425	1829848..1830147	+	300	WP_009883720.1	HigA family addiction module antidote protein	Antitoxin
CXR23_RS08430	1830181..1831371	+	1191	WP_096146801.1	PD-(D/E)XK nuclease family protein	-
CXR23_RS08435	1831448..1832488	-	1041	WP_035278961.1	methionine synthase	-
CXR23_RS08440	1832506..1833498	-	993	WP_069599929.1	DUF1852 domain-containing protein	-
CXR23_RS08445	1833624..1834565	+	942	WP_029417202.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1819271..1831371	12100
	-	flank	IS/Tn	-	-	1824804..1825997	1193

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-69)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10928.30 Da
• Isoelectric Point: 10.7346

>T90144 WP_009883719.1 NZ_CP025330:1829557-1829838 [Brevibacterium aurantiacum]
VIRSFADRDTERVWRREPARRLDPRIHKTANRKLHQLDAAVSLDSMRVPPGNRLESLRGDRAGQHSIRINDQWRICFRWT
GAGAEEVEIVDYH

Nucleotide

• Length: 282 bp

>T90144 NZ_CP025330:1829557-1829838 [Brevibacterium aurantiacum]
GTGATCAGATCGTTTGCCGACCGCGACACCGAGCGTGTGTGGCGTCGTGAACCGGCGAGGCGACTTGATCCTCGTATTCA
CAAGACTGCGAATCGGAAGTTGCATCAGCTGGATGCAGCGGTGTCGCTCGATTCCATGCGTGTTCCGCCCGGTAATCGTC
TGGAGTCACTCAGAGGTGATCGGGCTGGTCAGCATAGCATTCGCATCAACGATCAGTGGCGGATCTGCTTTAGGTGGACC
GGTGCCGGTGCAGAAGAAGTCGAGATCGTGGACTATCACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11132.84 Da
• Isoelectric Point: 5.7011

>AT90144 WP_009883720.1 NZ_CP025330:1829848-1830147 [Brevibacterium aurantiacum]
MSEKLYPPIHPGEVLMEDFIEGFGITQNKLAVSIGVPPRRINEIVHGKRAITADTALRLGRYFGVEPQFWLNLQSRYDLE
LAEERVSDQVAAITPLKVA

Nucleotide

• Length: 300 bp

>AT90144 NZ_CP025330:1829848-1830147 [Brevibacterium aurantiacum]
ATGTCCGAGAAGCTTTACCCGCCGATCCATCCGGGGGAAGTCCTCATGGAGGATTTCATCGAGGGGTTCGGTATCACGCA
GAATAAGCTCGCTGTGTCGATCGGTGTGCCACCTCGGCGTATCAATGAAATAGTGCACGGAAAGCGTGCCATCACTGCCG
ACACAGCTTTGCGTTTGGGGCGGTATTTCGGTGTGGAGCCGCAATTCTGGCTTAACCTGCAGAGTCGTTATGATCTGGAG
CTTGCAGAGGAACGCGTGTCCGACCAGGTCGCGGCAATCACGCCGCTTAAGGTTGCGTAA

Structures

Toxin

• AlphaFold DB: A0A2A3ZLA3