Detailed information of TA system TA090139

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 229903..230157
• Replicon: plasmid unnamed
• Accession: NZ_CP025329
• Organism: Escherichia coli strain ExPEC XM

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CXG97_RS28145
• Protein ID: WP_001351576.1
• Coordinates: 229951..230157 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	229903..229964 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXG97_RS28120	226651..227397	+	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-
CXG97_RS28125	227456..228316	+	861	WP_000704513.1	alpha/beta hydrolase	-
CXG97_RS28130	228419..228979	+	561	WP_000139315.1	fertility inhibition protein FinO	-
CXG97_RS28135	229115..229327	+	213	WP_001299730.1	ANR family transcriptional regulator	-
CXG97_RS29065	229573..229647	+	75	Protein_236	endonuclease	-
-	229903..229964	-	62	NuclAT_2	-	Antitoxin
-	229903..229964	-	62	NuclAT_2	-	Antitoxin
-	229903..229964	-	62	NuclAT_2	-	Antitoxin
-	229903..229964	-	62	NuclAT_2	-	Antitoxin
CXG97_RS28145	229951..230157	+	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
CXG97_RS28150	230441..230698	+	258	WP_000083845.1	replication regulatory protein RepA	-
CXG97_RS28160	230930..231004	+	75	WP_001442103.1	RepA leader peptide Tap	-
CXG97_RS28165	230997..231479	+	483	WP_001273588.1	hypothetical protein	-
CXG97_RS28170	231472..232329	+	858	WP_019842754.1	incFII family plasmid replication initiator RepA	-
CXG97_RS28175	232626..234203	-	1578	WP_001323889.1	PAS domain-containing methyl-accepting chemotaxis protein	-
CXG97_RS28180	234513..235073	+	561	WP_001161490.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / aph(6)-Id / aph(3'')-Ib / tet(A)	vat / iroN / iroE / iroD / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..286854	286854
	-	inside	IScluster/Tn	tet(A)	-	234513..249115	14602

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T90139 WP_001351576.1 NZ_CP025329:229951-230157 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T90139 NZ_CP025329:229951-230157 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT90139 NZ_CP025329:c229964-229903 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin