Detailed information of TA system TA090135

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 191814..192083
• Replicon: plasmid unnamed
• Accession: NZ_CP025329
• Organism: Escherichia coli strain ExPEC XM

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CXG97_RS27900
• Protein ID: WP_001312861.1
• Coordinates: 191925..192083 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	191814..191879 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXG97_RS27870	187589..188128	+	540	WP_009426937.1	single-stranded DNA-binding protein	-
CXG97_RS27875	188184..188417	+	234	WP_000006030.1	DUF905 family protein	-
CXG97_RS27880	188482..190440	+	1959	WP_101357017.1	ParB/RepB/Spo0J family partition protein	-
CXG97_RS27885	190495..190929	+	435	WP_000845930.1	conjugation system SOS inhibitor PsiB	-
CXG97_RS27890	190926..191645	+	720	WP_021573167.1	plasmid SOS inhibition protein A	-
-	191657..191881	+	225	NuclAT_0	-	-
-	191657..191881	+	225	NuclAT_0	-	-
-	191657..191881	+	225	NuclAT_0	-	-
-	191657..191881	+	225	NuclAT_0	-	-
-	191814..191879	+	66	-	-	Antitoxin
CXG97_RS27900	191925..192083	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CXG97_RS29050	192437..192649	-	213	WP_162491396.1	hypothetical protein	-
CXG97_RS27920	193005..193292	+	288	WP_101357019.1	hypothetical protein	-
CXG97_RS27925	193411..194232	+	822	WP_145607291.1	DUF945 domain-containing protein	-
CXG97_RS27930	194529..195176	-	648	WP_000614934.1	transglycosylase SLT domain-containing protein	-
CXG97_RS27935	195442..195825	+	384	WP_000124981.1	relaxosome protein TraM	-
CXG97_RS27940	196016..196702	+	687	WP_000332484.1	PAS domain-containing protein	-
CXG97_RS27945	196796..197023	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / aph(6)-Id / aph(3'')-Ib / tet(A)	vat / iroN / iroE / iroD / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..286854	286854

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T90135 WP_001312861.1 NZ_CP025329:191925-192083 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T90135 NZ_CP025329:191925-192083 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT90135 NZ_CP025329:191814-191879 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin