Detailed information of TA system TA090121

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4459303..4459905
• Replicon: chromosome
• Accession: NZ_CP025328
• Organism: Escherichia coli strain ExPEC XM

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: CXG97_RS23240
• Protein ID: WP_000897302.1
• Coordinates: 4459303..4459614 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CXG97_RS23245
• Protein ID: WP_000356397.1
• Coordinates: 4459615..4459905 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXG97_RS23215	4455217..4455816	+	600	WP_001314324.1	glucose-1-phosphatase	-
CXG97_RS23220	4455810..4456682	+	873	WP_000920754.1	virulence factor BrkB family protein	-
CXG97_RS23225	4456679..4457116	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CXG97_RS23230	4457161..4458102	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CXG97_RS23235	4458166..4459074	-	909	WP_001331553.1	alpha/beta hydrolase	-
CXG97_RS23240	4459303..4459614	+	312	WP_000897302.1	hypothetical protein	Toxin
CXG97_RS23245	4459615..4459905	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CXG97_RS23255	4460264..4460542	+	279	WP_001296612.1	hypothetical protein	-
CXG97_RS23265	4460938..4461156	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
CXG97_RS23270	4461372..4462301	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
CXG97_RS23275	4462298..4462933	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CXG97_RS23280	4462930..4463832	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T90121 WP_000897302.1 NZ_CP025328:4459303-4459614 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T90121 NZ_CP025328:4459303-4459614 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT90121 WP_000356397.1 NZ_CP025328:4459615-4459905 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT90121 NZ_CP025328:4459615-4459905 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416