Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1651482..1652044 Replicon chromosome
Accession NZ_CP025328
Organism Escherichia coli strain ExPEC XM

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag CXG97_RS08890 Protein ID WP_000605675.1
Coordinates 1651766..1652044 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag CXG97_RS08885 Protein ID WP_000781370.1
Coordinates 1651482..1651766 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXG97_RS08870 1646663..1649710 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
CXG97_RS08875 1649723..1650607 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
CXG97_RS08880 1650600..1651253 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
CXG97_RS08885 1651482..1651766 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
CXG97_RS08890 1651766..1652044 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CXG97_RS08895 1652230..1653240 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
CXG97_RS08900 1653374..1655071 - 1698 WP_000433462.1 malate dehydrogenase -
CXG97_RS08905 1655227..1655364 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
CXG97_RS08910 1655466..1655681 - 216 WP_000495766.1 biofilm-dependent modulation protein -
CXG97_RS08920 1656026..1656457 + 432 WP_000152310.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T90107 WP_000605675.1 NZ_CP025328:c1652044-1651766 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T90107 NZ_CP025328:c1652044-1651766 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT90107 WP_000781370.1 NZ_CP025328:c1651766-1651482 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT90107 NZ_CP025328:c1651766-1651482 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References