Detailed information of TA system TA090096

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4853382..4853984
• Replicon: chromosome
• Accession: NZ_CP025318
• Organism: Escherichia coli strain FORC_042

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FORC42_RS25645
• Protein ID: WP_000897305.1
• Coordinates: 4853673..4853984 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FORC42_RS25640
• Protein ID: WP_000356397.1
• Coordinates: 4853382..4853672 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC42_RS25605	4848929..4849564	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FORC42_RS25610	4849561..4850490	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
FORC42_RS25620	4850889..4851869	+	981	WP_000399676.1	IS110-like element IS621 family transposase	-
FORC42_RS25625	4852098..4852340	-	243	WP_001086388.1	hypothetical protein	-
FORC42_RS25630	4852559..4852777	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
FORC42_RS25640	4853382..4853672	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
FORC42_RS25645	4853673..4853984	-	312	WP_000897305.1	hypothetical protein	Toxin
FORC42_RS25650	4854213..4855121	+	909	WP_001162704.1	alpha/beta hydrolase	-
FORC42_RS25655	4855185..4856126	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
FORC42_RS25660	4856171..4856608	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FORC42_RS25665	4856605..4857478	-	874	Protein_4684	virulence factor BrkB family protein	-
FORC42_RS25670	4857472..4858071	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4850889..4851869	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T90096 WP_000897305.1 NZ_CP025318:c4853984-4853673 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T90096 NZ_CP025318:c4853984-4853673 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT90096 WP_000356397.1 NZ_CP025318:c4853672-4853382 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT90096 NZ_CP025318:c4853672-4853382 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7