Detailed information of TA system TA090093

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 4016769..4017310
• Replicon: chromosome
• Accession: NZ_CP025318
• Organism: Escherichia coli strain FORC_042

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: U9XPG0
• Locus tag: FORC42_RS21510
• Protein ID: WP_000615976.1
• Coordinates: 4017032..4017310 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: FORC42_RS21505
• Protein ID: WP_024216090.1
• Coordinates: 4016769..4017029 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC42_RS21480	4012552..4013337	-	786	WP_162733463.1	putative lateral flagellar export/assembly protein LafU	-
FORC42_RS21485	4013309..4015021	+	1713	Protein_3937	flagellar biosynthesis protein FlhA	-
FORC42_RS21490	4015126..4015404	+	279	WP_024216089.1	type II toxin-antitoxin system HicA family toxin	-
FORC42_RS21495	4015397..4015753	+	357	WP_001030486.1	type II toxin-antitoxin system HicB family antitoxin	-
FORC42_RS21500	4015810..4016583	-	774	WP_000543897.1	C40 family peptidase	-
FORC42_RS21505	4016769..4017029	+	261	WP_024216090.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
FORC42_RS21510	4017032..4017310	+	279	WP_000615976.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
FORC42_RS21515	4017466..4018206	+	741	WP_001225679.1	murein L,D-transpeptidase	-
FORC42_RS21520	4018177..4018944	-	768	WP_000333380.1	class II glutamine amidotransferase	-
FORC42_RS21525	4019150..4019728	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10800.59 Da
• Isoelectric Point: 10.0702

>T90093 WP_000615976.1 NZ_CP025318:4017032-4017310 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T90093 NZ_CP025318:4017032-4017310 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9490.91 Da
• Isoelectric Point: 5.8275

>AT90093 WP_024216090.1 NZ_CP025318:4016769-4017029 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLARMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT90093 NZ_CP025318:4016769-4017029 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCCGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: U9XPG0