Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1924799..1925301 Replicon chromosome
Accession NZ_CP025318
Organism Escherichia coli strain FORC_042

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FORC42_RS10025 Protein ID WP_000767829.1
Coordinates 1925047..1925301 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FORC42_RS10020 Protein ID WP_001259255.1
Coordinates 1924799..1925050 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC42_RS09990 1919977..1921044 - 1068 WP_021533029.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FORC42_RS09995 1921044..1922114 - 1071 WP_021533028.1 histidinol-phosphate transaminase -
FORC42_RS10000 1922111..1923415 - 1305 WP_021533027.1 histidinol dehydrogenase -
FORC42_RS10005 1923421..1924320 - 900 WP_104951114.1 ATP phosphoribosyltransferase -
FORC42_RS10015 1924466..1924516 - 51 WP_001364200.1 his operon leader peptide -
FORC42_RS10020 1924799..1925050 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FORC42_RS10025 1925047..1925301 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FORC42_RS10030 1925384..1926208 + 825 WP_000754758.1 SDR family oxidoreductase -
FORC42_RS10035 1926254..1927183 + 930 WP_000803351.1 LysR family transcriptional regulator -
FORC42_RS10040 1927398..1927460 + 63 WP_010723108.1 membrane protein YoeI -
FORC42_RS10045 1927450..1928808 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FORC42_RS10050 1928987..1930045 + 1059 WP_104951115.1 transport protein YeeE -
FORC42_RS10055 1930059..1930286 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T90081 WP_000767829.1 NZ_CP025318:1925047-1925301 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T90081 NZ_CP025318:1925047-1925301 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTTTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT90081 WP_001259255.1 NZ_CP025318:1924799-1925050 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT90081 NZ_CP025318:1924799-1925050 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCTCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References