Detailed information of TA system TA090031

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4043663..4044251
• Replicon: chromosome
• Accession: NZ_CP025298
• Organism: Stenotrophomonas maltophilia strain CSM2

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A397NGT4
• Locus tag: SmaCSM2_RS18530
• Protein ID: WP_049444941.1
• Coordinates: 4043973..4044251 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SmaCSM2_RS18525
• Protein ID: WP_006471116.1
• Coordinates: 4043663..4043962 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SmaCSM2_RS18505	4039128..4040315	+	1188	WP_049445499.1	pyridoxal phosphate-dependent aminotransferase	-
SmaCSM2_RS18510	4040403..4042499	-	2097	WP_101766106.1	prolyl oligopeptidase family protein	-
SmaCSM2_RS18515	4042569..4042892	-	324	WP_049444946.1	hypothetical protein	-
SmaCSM2_RS18520	4043020..4043622	+	603	WP_014038542.1	lipocalin family protein	-
SmaCSM2_RS18525	4043663..4043962	-	300	WP_006471116.1	HigA family addiction module antidote protein	Antitoxin
SmaCSM2_RS18530	4043973..4044251	-	279	WP_049444941.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SmaCSM2_RS18535	4044447..4045136	-	690	WP_014038544.1	ABC transporter permease	-
SmaCSM2_RS18540	4045133..4046140	-	1008	WP_014038545.1	methionine ABC transporter ATP-binding protein	-
SmaCSM2_RS18545	4046351..4047247	-	897	WP_049444938.1	DMT family transporter	-
SmaCSM2_RS18550	4047434..4047916	+	483	WP_014038547.1	YajQ family cyclic di-GMP-binding protein	-
SmaCSM2_RS18555	4048018..4048584	+	567	WP_032129027.1	DUF1415 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (23-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10713.25 Da
• Isoelectric Point: 10.8303

>T90031 WP_049444941.1 NZ_CP025298:c4044251-4043973 [Stenotrophomonas maltophilia]
MIRSFRCKQTRALFEGTCPRAWRQIRRVAERKLQLLDSAQTLAFLRSPPGNRLEALSGDRRGQYSLRISNQWRLCFTWTD
GGADAVEIVDYH

Nucleotide

• Length: 279 bp

>T90031 NZ_CP025298:c4044251-4043973 [Stenotrophomonas maltophilia]
ATGATCCGTTCATTCCGCTGCAAGCAGACCCGCGCCCTGTTTGAAGGAACCTGCCCACGCGCCTGGCGCCAGATCCGGCG
AGTGGCGGAACGCAAACTGCAGCTGCTCGACTCGGCACAGACGTTGGCCTTCCTGCGCAGCCCGCCCGGGAATCGTCTTG
AGGCGCTGTCAGGCGATCGAAGAGGGCAGTACAGCCTTCGCATCAGCAATCAATGGCGGCTCTGCTTTACCTGGACCGAC
GGCGGCGCCGATGCCGTGGAAATTGTTGACTATCACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10869.59 Da
• Isoelectric Point: 11.3450

>AT90031 WP_006471116.1 NZ_CP025298:c4043962-4043663 [Stenotrophomonas maltophilia]
MKRPHNGMRAIHPGEILREEFLLPLALSVNALAKALDVPATRLHAIVHGQRSISADTAARLARHFGGDAASWLAMQAAYD
LKTLPTRSEIERRVQPRAA

Nucleotide

• Length: 300 bp

>AT90031 NZ_CP025298:c4043962-4043663 [Stenotrophomonas maltophilia]
ATGAAGCGTCCCCACAACGGCATGCGCGCCATCCATCCTGGCGAGATCCTGCGCGAAGAGTTCCTGCTGCCCCTGGCGCT
GAGCGTGAATGCGCTGGCGAAGGCCCTGGATGTGCCCGCAACGCGACTGCACGCCATCGTGCACGGCCAACGCAGCATTT
CGGCGGACACTGCAGCGCGGCTGGCGCGGCATTTTGGTGGGGATGCCGCGTCCTGGCTCGCGATGCAGGCGGCCTACGAT
CTCAAGACATTGCCAACGCGCAGCGAAATCGAACGCCGGGTGCAGCCACGCGCGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A397NGT4