Detailed information of TA system TA090011

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3170155..3170848
• Replicon: chromosome
• Accession: NZ_CP025268
• Organism: Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: CXP41_RS16495
• Protein ID: WP_000415584.1
• Coordinates: 3170552..3170848 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: CXP41_RS16490
• Protein ID: WP_000650107.1
• Coordinates: 3170155..3170550 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXP41_RS16475	3166019..3168277	-	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-
CXP41_RS16480	3168415..3170022	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
CXP41_RS16490	3170155..3170550	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
CXP41_RS16495	3170552..3170848	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
CXP41_RS16500	3171053..3171535	-	483	WP_000183505.1	transcriptional regulator	-
CXP41_RS16505	3171588..3171980	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
CXP41_RS16510	3172132..3172791	+	660	WP_001221495.1	two-component system response regulator QseB	-
CXP41_RS16515	3172788..3174137	+	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
CXP41_RS16520	3174183..3174515	-	333	WP_000917684.1	DUF2645 family protein	-
CXP41_RS16530	3174834..3175415	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
CXP41_RS16535	3175446..3175760	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T90011 WP_000415584.1 NZ_CP025268:c3170848-3170552 [Escherichia coli str. K-12 substr. MG1655]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T90011 NZ_CP025268:c3170848-3170552 [Escherichia coli str. K-12 substr. MG1655]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT90011 WP_000650107.1 NZ_CP025268:c3170550-3170155 [Escherichia coli str. K-12 substr. MG1655]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT90011 NZ_CP025268:c3170550-3170155 [Escherichia coli str. K-12 substr. MG1655]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65