Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2093250..2093752 Replicon chromosome
Accession NZ_CP025268
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CXP41_RS11155 Protein ID WP_000767829.1
Coordinates 2093250..2093504 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CXP41_RS11160 Protein ID WP_001259255.1
Coordinates 2093501..2093752 (-) Length 84 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXP41_RS11125 2088265..2088492 - 228 WP_000234896.1 sulfurtransferase TusA -
CXP41_RS11130 2088506..2089564 - 1059 WP_000492339.1 transport protein YeeE -
CXP41_RS11135 2089743..2091101 - 1359 WP_101348614.1 putrescine/proton symporter PlaP -
CXP41_RS11140 2091091..2091153 - 63 WP_010723108.1 membrane protein YoeI -
CXP41_RS11145 2091368..2092297 - 930 WP_000803351.1 LysR family transcriptional regulator -
CXP41_RS11150 2092343..2093167 - 825 WP_000754737.1 SDR family oxidoreductase -
CXP41_RS11155 2093250..2093504 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CXP41_RS11160 2093501..2093752 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CXP41_RS11165 2094035..2094085 + 51 WP_001364200.1 his operon leader peptide -
CXP41_RS11175 2094231..2095130 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
CXP41_RS11180 2095136..2096440 + 1305 WP_000009594.1 histidinol dehydrogenase -
CXP41_RS11185 2096437..2097507 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
CXP41_RS11190 2097507..2098574 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-84)

Antitoxin

(1-73)


Sequences


Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T90006 WP_000767829.1 NZ_CP025268:c2093504-2093250 [Escherichia coli str. K-12 substr. MG1655]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T90006 NZ_CP025268:c2093504-2093250 [Escherichia coli str. K-12 substr. MG1655]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT90006 WP_001259255.1 NZ_CP025268:c2093752-2093501 [Escherichia coli str. K-12 substr. MG1655]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT90006 NZ_CP025268:c2093752-2093501 [Escherichia coli str. K-12 substr. MG1655]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q


Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References