Detailed information of TA system TA089981

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 251834..252528
• Replicon: chromosome
• Accession: NZ_CP025268
• Organism: Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: CXP41_RS01225
• Protein ID: WP_001263489.1
• Coordinates: 252130..252528 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: CXP41_RS01220
• Protein ID: WP_000554758.1
• Coordinates: 251834..252127 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXP41_RS01200	247466..247963	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
CXP41_RS01205	248187..249899	-	1713	Protein_225	flagellar biosynthesis protein FlhA	-
CXP41_RS01210	249871..250656	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
CXP41_RS01215	250727..251782	+	1056	WP_001226164.1	DNA polymerase IV	-
CXP41_RS01220	251834..252127	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CXP41_RS01225	252130..252528	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CXP41_RS01230	252538..252990	+	453	WP_101348542.1	GNAT family N-acetyltransferase	-
CXP41_RS01235	253308..253514	+	207	Protein_231	RtcB family protein	-
CXP41_RS01240	253510..254031	+	522	Protein_232	peptide chain release factor H	-
CXP41_RS01245	254088..255545	-	1458	WP_101348543.1	cytosol nonspecific dipeptidase	-
CXP41_RS01250	255806..256264	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	256860..256940	+	81	NuclAT_12	-	-
-	256860..256940	+	81	NuclAT_12	-	-
-	256860..256940	+	81	NuclAT_12	-	-
-	256860..256940	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T89981 WP_001263489.1 NZ_CP025268:252130-252528 [Escherichia coli str. K-12 substr. MG1655]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T89981 NZ_CP025268:252130-252528 [Escherichia coli str. K-12 substr. MG1655]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT89981 WP_000554758.1 NZ_CP025268:251834-252127 [Escherichia coli str. K-12 substr. MG1655]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT89981 NZ_CP025268:251834-252127 [Escherichia coli str. K-12 substr. MG1655]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3