Detailed information of TA system TA089979

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 42095..42753
• Replicon: plasmid pSMC_AB_BL01_1
• Accession: NZ_CP025267
• Organism: Acinetobacter baumannii isolate SMC_Paed_Ab_BL01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CV094_RS19585
• Protein ID: WP_000312250.1
• Coordinates: 42394..42753 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CV094_RS19580
• Protein ID: WP_001096429.1
• Coordinates: 42095..42394 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CV094_RS19535	37262..37891	+	630	WP_002081923.1	hypothetical protein	-
CV094_RS19540	37931..38440	+	510	WP_001043199.1	hypothetical protein	-
CV094_RS19545	38522..39034	+	513	WP_000840065.1	hypothetical protein	-
CV094_RS19550	39078..39419	+	342	WP_002001471.1	hypothetical protein	-
CV094_RS19555	39456..40184	+	729	WP_001022819.1	hypothetical protein	-
CV094_RS19565	40419..40972	+	554	Protein_53	hypothetical protein	-
CV094_RS19570	41022..41558	+	537	WP_060853649.1	hypothetical protein	-
CV094_RS19580	42095..42394	-	300	WP_001096429.1	helix-turn-helix transcriptional regulator	Antitoxin
CV094_RS19585	42394..42753	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CV094_RS19590	42954..43520	+	567	WP_031948745.1	hypothetical protein	-
CV094_RS19595	43569..43751	+	183	WP_000373384.1	hypothetical protein	-
CV094_RS19600	43818..44516	+	699	WP_000873188.1	hypothetical protein	-
CV094_RS19605	44655..45038	+	384	WP_000654347.1	hypothetical protein	-
CV094_RS19610	45105..45863	+	759	WP_060853650.1	hypothetical protein	-
CV094_RS19615	45915..46250	+	336	WP_000654055.1	hypothetical protein	-
CV094_RS19625	47115..47429	+	315	WP_000708713.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..74241	74241

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T89979 WP_000312250.1 NZ_CP025267:c42753-42394 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T89979 NZ_CP025267:c42753-42394 [Acinetobacter baumannii]
ATGGCTTGGGATGTAGAAACAACAGAACTTTTTGATTCTTGGTTGGCAGAACAAGATGAAAATGCTCAAGATAAAATCTT
AGCGTCTTTGCTTGTTTTGTCAGAACTGGGGCCAAATCTAGGCCGGCCTCATGTTGATACTATTAAAGAATCAAAATATC
CGAACATGAAAGAGATACGTGTGCAAGTTAAAGGTCATCCGATCCGTGGTTTCTTTGCATTTGATCCAGAACGTAAAGCC
ATTGTTTTGTGTGCTGGAGATAAGAAAGGGCTAAATGAAAAAGCCTTTTATAAAGAAATGATCAAGATTGCAGATGAACA
ATATGAGCAATATTTACGTGATAATTACGGAGATAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT89979 WP_001096429.1 NZ_CP025267:c42394-42095 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT89979 NZ_CP025267:c42394-42095 [Acinetobacter baumannii]
ATGGCTAAATTAAACGATATAATTTCAAAACGCTCACCTGAAAGCCAACAACGCATCAAGAAAATGGCAAATCAAATGCT
ATTGGATATTAACCTATCGAACCTACGGGAAGAGTTAAAAATATCTCAAGCTGAAATTGCAAAAAATCTAGGTGTAAGCC
AAGCTGCTATTTCAAAACGTGAAAAATTAGGTAAAGATCTTAAGATTTCATCATTAAAAGAATATGTTGAAGCTATGGGT
GGCAAAGTAAGGTTAGATGTTGAGTTACCTAACGGAAAGCATGTTGCTATATCTATTTAA