Detailed information of TA system TA089966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-couple_hipB
• Location: 1071285..1071933
• Replicon: chromosome
• Accession: NZ_CP025256
• Organism: Streptococcus pneumoniae strain Xen35

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0H2UQ08
• Locus tag: CXP32_RS05690
• Protein ID: WP_000551587.1
• Coordinates: 1071285..1071650 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0H2UQ20
• Locus tag: CXP32_RS05695
• Protein ID: WP_000794247.1
• Coordinates: 1071640..1071933 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXP32_RS05650	1066524..1067141	+	618	WP_000424676.1	Rha family transcriptional regulator	-
CXP32_RS05655	1067176..1067628	+	453	WP_000141102.1	hypothetical protein	-
CXP32_RS05660	1067625..1068071	+	447	WP_000171242.1	DnaD domain protein	-
CXP32_RS05665	1068083..1068967	+	885	WP_000838905.1	ATP-binding protein	-
CXP32_RS05670	1069216..1069866	+	651	WP_000138311.1	hypothetical protein	-
CXP32_RS05675	1069913..1070341	+	429	WP_000918210.1	hypothetical protein	-
CXP32_RS05680	1070349..1070660	+	312	WP_001113833.1	hypothetical protein	-
CXP32_RS05685	1070678..1071088	+	411	WP_000162610.1	hypothetical protein	-
CXP32_RS05690	1071285..1071650	+	366	WP_000551587.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CXP32_RS05695	1071640..1071933	+	294	WP_000794247.1	helix-turn-helix transcriptional regulator	Antitoxin
CXP32_RS05700	1071951..1073141	+	1191	WP_000123149.1	hypothetical protein	-
CXP32_RS12060	1073144..1073320	+	177	WP_000346914.1	hypothetical protein	-
CXP32_RS05705	1073640..1074086	-	447	WP_061631657.1	tyrosine-type recombinase/integrase	-
CXP32_RS05710	1073986..1074831	-	846	Protein_1064	IS630 family transposase	-
CXP32_RS05720	1075002..1075157	+	156	Protein_1065	diacylglycerol kinase family lipid kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1063231..1119540	56309

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (35-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-88)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 14514.67 Da
• Isoelectric Point: 9.8086

>T89966 WP_000551587.1 NZ_CP025256:1071285-1071650 [Streptococcus pneumoniae]
VHNIYFYKDKNGNEPVFDYMRELTSKKGKDSRIKLNKINDYIELLSQHGTRAGEPYIKHLDAEIWELRPLRDRILFVAWM
DGSFVLLHHFMKRTQKTPKREIEQAKRELADLKERGLDNEK

Nucleotide

• Length: 366 bp

>T89966 NZ_CP025256:1071285-1071650 [Streptococcus pneumoniae]
GTGCATAATATCTATTTTTACAAGGATAAAAACGGCAATGAGCCTGTTTTTGATTATATGCGAGAGCTTACCAGTAAAAA
AGGGAAAGATAGCAGAATTAAGCTTAATAAAATTAATGATTATATTGAGTTGTTAAGCCAACATGGAACACGCGCAGGCG
AACCATATATTAAGCATTTAGATGCTGAAATTTGGGAGCTGAGACCACTTAGAGATAGAATTTTATTTGTTGCTTGGATG
GATGGTAGTTTTGTTTTACTGCATCATTTTATGAAAAGGACACAGAAAACACCCAAAAGAGAGATTGAGCAAGCTAAACG
TGAACTTGCAGATTTAAAAGAAAGAGGTTTAGACAATGAAAAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10751.45 Da
• Isoelectric Point: 5.1991

>AT89966 WP_000794247.1 NZ_CP025256:1071640-1071933 [Streptococcus pneumoniae]
MKNNAIGSNWKDVRAELFSKEEILESDMRVAIMSELIEARNEKGISQKKLEEMSGVSQPVIARMETGKTSPQLDTVLKVL
ASLGKTLAVVPLEHEQV

Nucleotide

• Length: 294 bp

>AT89966 NZ_CP025256:1071640-1071933 [Streptococcus pneumoniae]
ATGAAAAATAATGCTATTGGTAGTAACTGGAAAGATGTAAGAGCTGAATTATTCAGCAAAGAGGAAATTTTGGAAAGTGA
TATGCGCGTGGCTATCATGAGTGAGCTTATCGAGGCTAGAAATGAAAAGGGTATTAGTCAGAAGAAACTAGAGGAGATGA
GCGGTGTTAGTCAGCCAGTTATCGCTAGAATGGAAACAGGAAAGACAAGCCCACAACTTGATACAGTATTAAAAGTGTTG
GCAAGTCTTGGTAAGACCTTAGCTGTTGTACCATTAGAGCATGAGCAGGTTTAG

Structures

Toxin

• PDB: 6AF3
• PDB: 6AF4

Antitoxin

• AlphaFold DB: A0A0H2UQ20