Detailed information of TA system TA089961

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 50483..50753
• Replicon: plasmid pBH100-1
• Accession: NZ_CP025253
• Organism: Escherichia coli strain BH100 substr. MG2017

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BH100B_RS26685
• Protein ID: WP_001312861.1
• Coordinates: 50595..50753 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	50483..50546 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BH100B_RS26650	46105..46698	-	594	WP_000428546.1	tetracyline resistance-associated transcriptional repressor TetC	-
BH100B_RS26655	46831..47202	+	372	WP_035689358.1	helix-turn-helix domain-containing protein	-
BH100B_RS26660	47212..48419	-	1208	Protein_53	IS4 family transposase	-
BH100B_RS26670	48529..49110	+	582	Protein_54	hypothetical protein	-
BH100B_RS26675	49165..49599	+	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
BH100B_RS26680	49596..50315	+	720	WP_000116348.1	plasmid SOS inhibition protein A	-
-	50327..50551	+	225	NuclAT_0	-	-
-	50327..50551	+	225	NuclAT_0	-	-
-	50327..50551	+	225	NuclAT_0	-	-
-	50327..50551	+	225	NuclAT_0	-	-
BH100B_RS27295	50336..50515	-	180	WP_001309233.1	hypothetical protein	-
-	50483..50546	-	64	-	-	Antitoxin
BH100B_RS26685	50595..50753	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
BH100B_RS27455	50991..51368	-	378	Protein_59	hypothetical protein	-
BH100B_RS26705	51668..51964	+	297	WP_001272251.1	hypothetical protein	-
BH100B_RS26710	52075..52896	+	822	WP_001234445.1	DUF945 domain-containing protein	-
BH100B_RS26715	53193..53795	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
BH100B_RS26725	54118..54501	+	384	WP_001354030.1	relaxosome protein TraM	-
BH100B_RS26730	54695..55366	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
BH100B_RS26735	55503..55730	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / ant(3'')-Ia / catA1 / aph(3')-Ia / tet(B) / blaTEM-1A	-	1..105801	105801

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T89961 WP_001312861.1 NZ_CP025253:50595-50753 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T89961 NZ_CP025253:50595-50753 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT89961 NZ_CP025253:c50546-50483 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin