Detailed information of TA system TA089957

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-agrB/SymE(toxin)
• Location: 5027063..5027475
• Replicon: chromosome
• Accession: NZ_CP025251
• Organism: Escherichia coli strain BH100 substr. MG2017

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: BH100B_RS25520
• Protein ID: WP_000132614.1
• Coordinates: 5027063..5027404 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	5027399..5027475 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BH100B_RS25500	5022132..5023412	-	1281	WP_001298033.1	DUF445 domain-containing protein	-
BH100B_RS25505	5023597..5024517	+	921	WP_000181195.1	Rpn family recombination-promoting nuclease/putative transposase	-
BH100B_RS25510	5024745..5024825	+	81	WP_020233658.1	hypothetical protein	-
BH100B_RS25515	5024923..5027016	+	2094	WP_000648237.1	DUF262 domain-containing protein	-
BH100B_RS25520	5027063..5027404	-	342	WP_000132614.1	endoribonuclease SymE	Toxin
-	5027399..5027475	+	77	NuclAT_6	-	Antitoxin
-	5027399..5027475	+	77	NuclAT_6	-	Antitoxin
-	5027399..5027475	+	77	NuclAT_6	-	Antitoxin
-	5027399..5027475	+	77	NuclAT_6	-	Antitoxin
-	5027399..5027475	+	77	NuclAT_7	-	Antitoxin
-	5027399..5027475	+	77	NuclAT_7	-	Antitoxin
-	5027399..5027475	+	77	NuclAT_7	-	Antitoxin
-	5027399..5027475	+	77	NuclAT_7	-	Antitoxin
BH100B_RS25525	5027625..5029331	-	1707	WP_001100194.1	restriction endonuclease subunit S	-
BH100B_RS25530	5029331..5030800	-	1470	WP_001540814.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T89957 WP_000132614.1 NZ_CP025251:c5027404-5027063 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T89957 NZ_CP025251:c5027404-5027063 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACTGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT89957 NZ_CP025251:5027399-5027475 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin