Detailed information of TA system TA089954

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4390485..4391087
• Replicon: chromosome
• Accession: NZ_CP025251
• Organism: Escherichia coli strain BH100 substr. MG2017

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: BH100B_RS22280
• Protein ID: WP_000897305.1
• Coordinates: 4390485..4390796 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BH100B_RS22285
• Protein ID: WP_000356397.1
• Coordinates: 4390797..4391087 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BH100B_RS22255	4386399..4386998	+	600	WP_001298594.1	glucose-1-phosphatase	-
BH100B_RS22260	4386992..4387864	+	873	WP_000920762.1	virulence factor BrkB family protein	-
BH100B_RS22265	4387861..4388298	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BH100B_RS22270	4388343..4389284	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
BH100B_RS22275	4389348..4390256	-	909	WP_001298596.1	alpha/beta hydrolase	-
BH100B_RS22280	4390485..4390796	+	312	WP_000897305.1	hypothetical protein	Toxin
BH100B_RS22285	4390797..4391087	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
BH100B_RS22295	4391692..4391910	+	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
BH100B_RS22300	4392125..4393054	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
BH100B_RS22305	4393051..4393686	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
BH100B_RS22310	4393683..4394585	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12172.16 Da
• Isoelectric Point: 9.7791

>T89954 WP_000897305.1 NZ_CP025251:4390485-4390796 [Escherichia coli]
ILFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T89954 NZ_CP025251:4390485-4390796 [Escherichia coli]
ATCCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT89954 WP_000356397.1 NZ_CP025251:4390797-4391087 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT89954 NZ_CP025251:4390797-4391087 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7