Detailed information of TA system TA089951

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3561923..3562722
• Replicon: chromosome
• Accession: NZ_CP025251
• Organism: Escherichia coli strain BH100 substr. MG2017

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: BH100B_RS18055
• Protein ID: WP_000347252.1
• Coordinates: 3562258..3562722 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: BH100B_RS18050
• Protein ID: WP_001296435.1
• Coordinates: 3561923..3562258 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BH100B_RS18035	3557708..3558478	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
BH100B_RS18040	3558494..3559828	-	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
BH100B_RS18045	3560203..3561774	+	1572	WP_001273939.1	galactarate dehydratase	-
BH100B_RS18050	3561923..3562258	+	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
BH100B_RS18055	3562258..3562722	+	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
BH100B_RS18060	3562777..3563586	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
BH100B_RS18070	3563835..3565115	+	1281	WP_000681930.1	tagatose-bisphosphate aldolase subunit KbaZ	-
BH100B_RS18075	3565138..3565611	+	474	WP_001298322.1	PTS N-acetylgalactosamine transporter subunit IIB	-
BH100B_RS18080	3565622..3566401	+	780	Protein_3309	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
BH100B_RS18085	3566391..3567269	+	879	WP_023152018.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
BH100B_RS18090	3567287..3567721	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T89951 WP_000347252.1 NZ_CP025251:3562258-3562722 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T89951 NZ_CP025251:3562258-3562722 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAGCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT89951 WP_001296435.1 NZ_CP025251:3561923-3562258 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT89951 NZ_CP025251:3561923-3562258 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5