Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1568173..1568735 Replicon chromosome
Accession NZ_CP025251
Organism Escherichia coli strain BH100 substr. MG2017

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag BH100B_RS08080 Protein ID WP_000605675.1
Coordinates 1568457..1568735 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag BH100B_RS08075 Protein ID WP_000781370.1
Coordinates 1568173..1568457 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BH100B_RS08060 1564978..1565862 + 885 WP_001240581.1 formate dehydrogenase N subunit beta -
BH100B_RS08065 1565855..1566508 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
BH100B_RS08070 1566657..1568005 + 1349 WP_099989771.1 IS3-like element IS1397 family transposase -
BH100B_RS08075 1568173..1568457 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
BH100B_RS08080 1568457..1568735 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BH100B_RS08085 1568921..1569931 - 1011 WP_000642380.1 alcohol dehydrogenase AdhP -
BH100B_RS08090 1570065..1571762 - 1698 WP_000433462.1 malate dehydrogenase -
BH100B_RS08095 1571919..1572056 - 138 WP_099989695.1 stationary-phase-induced ribosome-associated protein -
BH100B_RS08100 1572158..1572373 - 216 WP_000495766.1 biofilm-dependent modulation protein -
BH100B_RS08110 1572718..1573149 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T89936 WP_000605675.1 NZ_CP025251:c1568735-1568457 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T89936 NZ_CP025251:c1568735-1568457 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT89936 WP_000781370.1 NZ_CP025251:c1568457-1568173 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT89936 NZ_CP025251:c1568457-1568173 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References