Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 3466067..3466740 Replicon chromosome
Accession NZ_CP025225
Organism Enterobacter cancerogenus strain CR-Eb1

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag CWI88_RS16530 Protein ID WP_101738092.1
Coordinates 3466354..3466740 (-) Length 129 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag CWI88_RS16525 Protein ID WP_101738091.1
Coordinates 3466067..3466351 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CWI88_RS16500 3461793..3462461 + 669 WP_101738088.1 enterobactin synthase subunit EntD -
CWI88_RS16505 3462424..3463263 - 840 WP_101738089.1 metal ABC transporter permease -
CWI88_RS16510 3463260..3464117 - 858 WP_006177065.1 iron/manganese ABC transporter permease subunit SitC -
CWI88_RS16515 3464114..3464917 - 804 WP_101738090.1 iron/manganese ABC transporter ATP-binding protein SitB -
CWI88_RS16520 3464920..3465831 - 912 WP_006177063.1 metal ABC transporter substrate-binding protein -
CWI88_RS16525 3466067..3466351 - 285 WP_101738091.1 helix-turn-helix domain-containing protein Antitoxin
CWI88_RS16530 3466354..3466740 - 387 WP_101738092.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CWI88_RS16535 3466879..3468912 - 2034 WP_006177060.1 choline BCCT transporter BetT -
CWI88_RS16545 3469041..3469628 + 588 WP_042320939.1 transcriptional regulator BetI -
CWI88_RS16550 3469642..3471114 + 1473 WP_088207732.1 betaine-aldehyde dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(38-116)

Antitoxin

Cro/C1-type helix-turn-helix domain

(35-87)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14346.53 Da        Isoelectric Point: 9.7081

>T89908 WP_101738092.1 NZ_CP025225:c3466740-3466354 [Enterobacter cancerogenus]
MSHALEFIETSLFTRQIKSIASDDELKDLQKELIAWPDKGDVIQHTGGLRKIRMAAGSKGKRGGARVIYFLATEEVIYLI
MAYPKNTKDTLTETEKAQLKKLTSVLRNTASNALDERCTMYPNQVRGG

Download         Length: 387 bp

>T89908 NZ_CP025225:c3466740-3466354 [Enterobacter cancerogenus]
ATGAGCCACGCGTTAGAATTTATCGAGACGTCACTTTTTACCCGGCAGATCAAAAGTATTGCATCGGATGACGAATTAAA
GGATCTCCAAAAGGAACTCATTGCCTGGCCTGATAAAGGTGACGTGATCCAGCATACCGGAGGCTTGCGTAAAATAAGGA
TGGCCGCTGGGTCAAAGGGAAAGAGAGGTGGAGCCAGAGTCATTTACTTTTTGGCCACGGAAGAGGTGATTTATCTCATC
ATGGCCTATCCAAAAAATACGAAGGACACTCTGACAGAGACAGAGAAAGCACAGCTTAAAAAGCTCACCTCTGTTCTTAG
AAATACTGCTTCCAATGCCCTTGATGAGAGGTGTACTATGTACCCCAATCAGGTAAGAGGAGGTTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10506.99 Da        Isoelectric Point: 10.2562

>AT89908 WP_101738091.1 NZ_CP025225:c3466351-3466067 [Enterobacter cancerogenus]
MSIFEELKSSLEEAVEIHSGKKTPSRVTRYEVADVRAIREQLNVTQSEMAKALGTSVDTIKSWESKRRNPTGLAAKVLKA
IRDNPAFYSALARQ

Download         Length: 285 bp

>AT89908 NZ_CP025225:c3466351-3466067 [Enterobacter cancerogenus]
ATGAGTATTTTTGAAGAACTGAAATCTTCGCTTGAAGAGGCTGTTGAGATCCATAGCGGAAAAAAAACACCCTCCCGAGT
CACTCGCTATGAAGTCGCCGATGTTCGTGCAATCAGAGAACAGCTTAATGTTACGCAAAGCGAAATGGCAAAGGCGCTCG
GCACGAGCGTTGATACCATAAAAAGCTGGGAGTCTAAACGTCGTAACCCAACGGGGCTTGCAGCGAAAGTCCTTAAGGCG
ATTCGGGATAACCCTGCGTTTTACAGCGCGCTTGCTAGGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References