Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 4573567..4574153 Replicon chromosome
Accession NZ_CP025211
Organism Klebsiella pneumoniae strain HZW25

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag CW753_RS22965 Protein ID WP_002920800.1
Coordinates 4573785..4574153 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag CW753_RS22960 Protein ID WP_004174006.1
Coordinates 4573567..4573788 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CW753_RS22940 (CW753_23060) 4569724..4570650 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
CW753_RS22945 (CW753_23065) 4570647..4571924 + 1278 WP_134906098.1 branched chain amino acid ABC transporter permease LivM -
CW753_RS22950 (CW753_23070) 4571921..4572688 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
CW753_RS22955 (CW753_23075) 4572690..4573403 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
CW753_RS22960 (CW753_23080) 4573567..4573788 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CW753_RS22965 (CW753_23085) 4573785..4574153 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
CW753_RS22970 (CW753_23090) 4574426..4575742 + 1317 WP_032442539.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
CW753_RS22975 (CW753_23095) 4575849..4576736 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
CW753_RS22980 (CW753_23100) 4576733..4577578 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
CW753_RS22985 (CW753_23105) 4577580..4578650 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4568553..4579387 10834


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T89886 WP_002920800.1 NZ_CP025211:4573785-4574153 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T89886 NZ_CP025211:4573785-4574153 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT89886 WP_004174006.1 NZ_CP025211:4573567-4573788 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT89886 NZ_CP025211:4573567-4573788 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References