Detailed information of TA system TA089880

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2787192..2787789
• Replicon: chromosome
• Accession: NZ_CP025211
• Organism: Klebsiella pneumoniae strain HZW25

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: CW753_RS14080
• Protein ID: WP_004893639.1
• Coordinates: 2787472..2787789 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: CW753_RS14075
• Protein ID: WP_004142561.1
• Coordinates: 2787192..2787479 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CW753_RS14040 (CW753_14050)	2782214..2782555	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
CW753_RS14045 (CW753_14055)	2782586..2783701	-	1116	WP_020316605.1	MBL fold metallo-hydrolase	-
CW753_RS14050 (CW753_14060)	2783881..2784150	+	270	Protein_2703	helix-turn-helix domain containing protein	-
CW753_RS14055 (CW753_14065)	2784218..2785438	+	1221	WP_000343760.1	ISL3-like element ISKox3 family transposase	-
CW753_RS14060 (CW753_14070)	2785466..2785786	+	321	Protein_2705	TetR/AcrR family transcriptional regulator	-
CW753_RS14065 (CW753_14075)	2785786..2786154	+	369	WP_032421096.1	MmcQ/YjbR family DNA-binding protein	-
CW753_RS14070 (CW753_14080)	2786274..2786927	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
CW753_RS14075 (CW753_14085)	2787192..2787479	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
CW753_RS14080 (CW753_14090)	2787472..2787789	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CW753_RS14085 (CW753_14095)	2787974..2789017	-	1044	WP_032410657.1	DUF2157 domain-containing protein	-
CW753_RS14095 (CW753_14105)	2789682..2790548	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
CW753_RS14100 (CW753_14110)	2790657..2792084	+	1428	WP_009308097.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2784218..2785438	1220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T89880 WP_004893639.1 NZ_CP025211:c2787789-2787472 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T89880 NZ_CP025211:c2787789-2787472 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT89880 WP_004142561.1 NZ_CP025211:c2787479-2787192 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT89880 NZ_CP025211:c2787479-2787192 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1