Detailed information of TA system TA089863

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 2444348..2445051
• Replicon: chromosome
• Accession: NZ_CP025198
• Organism: Acidipropionibacterium virtanenii strain JS278

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: JS278_RS11320
• Protein ID: WP_114045286.1
• Coordinates: 2444348..2444629 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JS278_RS11325
• Protein ID: WP_114045287.1
• Coordinates: 2444731..2445051 (-)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JS278_RS11275	2439767..2440324	+	558	WP_114045277.1	dihydrofolate reductase family protein	-
JS278_RS11280	2440328..2440744	-	417	WP_114045278.1	type II toxin-antitoxin system VapC family toxin	-
JS278_RS11285	2440741..2441022	-	282	WP_114045279.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
JS278_RS11290	2441066..2441602	-	537	WP_114045280.1	GNAT family N-acetyltransferase	-
JS278_RS11295	2441666..2442160	+	495	WP_114045281.1	tetratricopeptide repeat protein	-
JS278_RS11300	2442178..2442987	-	810	WP_114045282.1	GNAT family N-acetyltransferase	-
JS278_RS11305	2443064..2443492	+	429	WP_114045283.1	GNAT family N-acetyltransferase	-
JS278_RS11310	2443608..2444042	-	435	WP_114045284.1	hypothetical protein	-
JS278_RS11315	2444039..2444338	-	300	WP_114045285.1	HigA family addiction module antidote protein	-
JS278_RS11320	2444348..2444629	-	282	WP_114045286.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JS278_RS11325	2444731..2445051	-	321	WP_114045287.1	HigA family addiction module antidote protein	Antitoxin
JS278_RS11330	2445026..2445307	-	282	Protein_2216	type II toxin-antitoxin system RelE/ParE family toxin	-
JS278_RS11335	2445470..2445667	+	198	WP_114045288.1	hypothetical protein	-
JS278_RS11340	2445664..2445999	+	336	WP_114046290.1	type II toxin-antitoxin system PemK/MazF family toxin	-
JS278_RS11345	2446145..2447218	-	1074	WP_114045289.1	redox-regulated ATPase YchF	-
JS278_RS11350	2447278..2448192	+	915	WP_114045290.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (13-103)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10897.41 Da
• Isoelectric Point: 9.3241

>T89863 WP_114045286.1 NZ_CP025198:c2444629-2444348 [Acidipropionibacterium virtanenii]
VIRSFADRGTERVWRRESAKRLDPRIHKVADRKLHLLDTATVLDAQRVPPGNRLVLLKGDRAEQHSIRINDQWRICFCWT
DAGPEDVQIVDYH

Nucleotide

• Length: 282 bp

>T89863 NZ_CP025198:c2444629-2444348 [Acidipropionibacterium virtanenii]
GTGATCAGATCGTTCGCTGACCGTGGCACCGAGCGGGTGTGGCGGCGTGAGTCGGCGAAGCGACTCGATCCGCGCATCCA
CAAGGTCGCCGACAGGAAGCTGCATCTGTTGGACACAGCGACAGTGCTGGACGCGCAGCGCGTGCCGCCCGGCAACCGTC
TGGTACTGCTCAAGGGTGACAGAGCCGAGCAGCACAGCATTCGCATCAACGATCAATGGCGGATCTGCTTTTGCTGGACT
GACGCCGGTCCCGAAGATGTGCAGATCGTCGACTACCACTGA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11955.74 Da
• Isoelectric Point: 7.2039

>AT89863 WP_114045287.1 NZ_CP025198:c2445051-2444731 [Acidipropionibacterium virtanenii]
MSNSSTTTETDLIEPIHPGEILMEDFIKGFGITQNKLAVAIGVPPRRINEIVHGKRGISADTAIRLARYFGTSEEFWMNL
QSNYELRIEWRALRDKVAAITPLRVA

Nucleotide

• Length: 321 bp

>AT89863 NZ_CP025198:c2445051-2444731 [Acidipropionibacterium virtanenii]
ATGTCGAACTCGTCGACTACCACTGAAACTGACCTGATCGAGCCCATCCATCCGGGAGAGATCCTCATGGAGGACTTCAT
CAAGGGCTTCGGGATCACACAGAACAAGCTGGCCGTGGCCATTGGCGTGCCGCCACGGCGGATCAACGAGATCGTGCACG
GGAAGCGGGGCATCTCGGCCGATACGGCGATCCGTCTGGCTCGTTACTTCGGAACCTCCGAGGAGTTCTGGATGAACCTC
CAGTCGAACTACGAGTTGCGGATCGAATGGCGAGCGCTGCGGGACAAGGTCGCTGCGATCACGCCGCTCCGGGTCGCGTG
A