Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 48030..48687 Replicon plasmid NR5632_p1
Accession NZ_CP025144
Organism Klebsiella pneumoniae strain NR5632

Toxin (Protein)


Gene name higB Uniprot ID U3PDC3
Locus tag CXB25_RS27460 Protein ID WP_000270043.1
Coordinates 48030..48380 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag CXB25_RS27465 Protein ID WP_000124640.1
Coordinates 48385..48687 (+) Length 101 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXB25_RS27420 44504..45001 - 498 WP_000062185.1 hypothetical protein -
CXB25_RS27425 45004..45492 - 489 WP_001273096.1 DUF1643 domain-containing protein -
CXB25_RS27430 45589..45924 + 336 WP_000683476.1 hypothetical protein -
CXB25_RS27435 45939..46409 - 471 WP_001281821.1 hypothetical protein -
CXB25_RS27440 46402..46773 - 372 WP_000516916.1 hypothetical protein -
CXB25_RS27445 46784..46978 - 195 WP_000343597.1 hypothetical protein -
CXB25_RS27455 47319..47867 - 549 WP_001061574.1 transcriptional regulator -
CXB25_RS27460 48030..48380 + 351 WP_000270043.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CXB25_RS27465 48385..48687 + 303 WP_000124640.1 XRE family transcriptional regulator Antitoxin
CXB25_RS27470 48714..49007 - 294 WP_001239997.1 chromosome segregation protein ParM -
CXB25_RS27475 49095..49367 - 273 WP_001043047.1 HU family DNA-binding protein -
CXB25_RS27480 49425..49970 - 546 WP_032413569.1 thermonuclease family protein -
CXB25_RS27490 50094..51074 - 981 WP_012569499.1 IS5-like element ISKpn26 family transposase -
CXB25_RS27500 51383..52240 - 858 WP_001167032.1 hypothetical protein -
CXB25_RS27505 52227..52457 - 231 WP_000972663.1 hypothetical protein -
CXB25_RS27510 52457..52975 - 519 WP_000210758.1 nitrite reductase -
CXB25_RS27515 52972..53418 - 447 WP_000919345.1 Fe3+-siderophore ABC transporter permease -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / blaKPC-33 / sul1 / qacE / aac(6')-33 / ant(2'')-Ia / blaTEM-1D - 1..204123 204123
- flank IS/Tn - - 50094..51074 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-106)

Antitoxin

(33-79)


Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 13328.21 Da        Isoelectric Point: 5.2421

>T89831 WP_000270043.1 NZ_CP025144:48030-48380 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Download         Length: 351 bp

>T89831 NZ_CP025144:48030-48380 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11006.73 Da        Isoelectric Point: 7.2036

>AT89831 WP_000124640.1 NZ_CP025144:48385-48687 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Download         Length: 303 bp

>AT89831 NZ_CP025144:48385-48687 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7G8AFB9


Antitoxin

Source ID Structure

References