Detailed information of TA system TA089810

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 48178..48835
• Replicon: plasmid KP1768_p1
• Accession: NZ_CP025141
• Organism: Klebsiella pneumoniae strain KP1768

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: CXB24_RS27440
• Protein ID: WP_000270043.1
• Coordinates: 48178..48528 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CXB24_RS27445
• Protein ID: WP_000124640.1
• Coordinates: 48533..48835 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXB24_RS27400	44652..45149	-	498	WP_000062185.1	hypothetical protein	-
CXB24_RS27405	45152..45640	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
CXB24_RS27410	45737..46072	+	336	WP_000683476.1	hypothetical protein	-
CXB24_RS27415	46087..46557	-	471	WP_001281821.1	hypothetical protein	-
CXB24_RS27420	46550..46921	-	372	WP_000516916.1	hypothetical protein	-
CXB24_RS27425	46932..47126	-	195	WP_000343597.1	hypothetical protein	-
CXB24_RS27435	47467..48015	-	549	WP_001061574.1	transcriptional regulator	-
CXB24_RS27440	48178..48528	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CXB24_RS27445	48533..48835	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
CXB24_RS27450	48862..49155	-	294	WP_001239997.1	chromosome segregation protein ParM	-
CXB24_RS27455	49243..49515	-	273	WP_001043047.1	HU family DNA-binding protein	-
CXB24_RS27460	49573..50118	-	546	WP_032413569.1	thermonuclease family protein	-
CXB24_RS27470	50242..51222	-	981	WP_012569499.1	IS5-like element ISKpn26 family transposase	-
CXB24_RS27480	51531..52388	-	858	WP_001167032.1	hypothetical protein	-
CXB24_RS27485	52375..52605	-	231	WP_000972663.1	hypothetical protein	-
CXB24_RS27490	52605..53123	-	519	WP_000210758.1	nitrite reductase	-
CXB24_RS27495	53120..53566	-	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / blaKPC-2 / sul1 / qacE / aac(6')-33 / ant(2'')-Ia / blaTEM-1D	-	1..204734	204734
	-	flank	IS/Tn	-	-	50242..51222	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T89810 WP_000270043.1 NZ_CP025141:48178-48528 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T89810 NZ_CP025141:48178-48528 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT89810 WP_000124640.1 NZ_CP025141:48533-48835 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT89810 NZ_CP025141:48533-48835 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9