Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 6704135..6704763 Replicon chromosome
Accession NZ_CP025113
Organism Bradyrhizobium sp. SK17 strain CBNU

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag CWS35_RS31120 Protein ID WP_100955268.1
Coordinates 6704135..6704419 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID -
Locus tag CWS35_RS31125 Protein ID WP_100955269.1
Coordinates 6704464..6704763 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CWS35_RS31110 6699266..6700111 + 846 WP_100955266.1 DUF3471 domain-containing protein -
CWS35_RS31115 6700123..6703911 - 3789 WP_100955267.1 hypothetical protein -
CWS35_RS31120 6704135..6704419 + 285 WP_100955268.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CWS35_RS31125 6704464..6704763 + 300 WP_100955269.1 HigA family addiction module antidote protein Antitoxin
CWS35_RS31130 6704806..6705609 - 804 WP_100955270.1 flagellar protein FlbB -
CWS35_RS31135 6705606..6706079 - 474 WP_024580963.1 hypothetical protein -
CWS35_RS31140 6706076..6707278 - 1203 WP_024580964.1 flagellar motor switch protein FliM -
CWS35_RS31145 6707297..6707788 - 492 WP_024580965.1 flagellar basal body-associated protein FliL -
CWS35_RS31150 6708170..6708934 + 765 WP_100955271.1 flagellar basal-body rod protein FlgF -
CWS35_RS31155 6708949..6709737 + 789 WP_024580967.1 flagellar basal-body rod protein FlgG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(11-65)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10805.20 Da        Isoelectric Point: 9.0347

>T89782 WP_100955268.1 NZ_CP025113:6704135-6704419 [Bradyrhizobium sp. SK17]
VIKSFKDAVTRAAFDGETRKGFPADLLKVARRKLQYLHAATTLQDLRSPPGNRLEALKGDRRDQHSIRVNDQFRICFIWT
EEGAVDVEFTDYHS

Download         Length: 285 bp

>T89782 NZ_CP025113:6704135-6704419 [Bradyrhizobium sp. SK17]
GTGATTAAGAGCTTCAAAGACGCTGTGACCAGAGCGGCCTTTGACGGCGAGACCCGAAAGGGATTTCCGGCCGACTTGCT
TAAAGTCGCGCGCCGCAAACTCCAGTATCTTCACGCCGCCACGACGTTGCAGGACCTTCGGTCTCCTCCTGGCAATCGGC
TGGAAGCTCTAAAGGGAGATCGCCGGGACCAACATTCAATCCGGGTGAACGATCAGTTCCGCATCTGTTTCATCTGGACC
GAAGAAGGTGCGGTGGACGTGGAATTCACGGACTACCACTCCTAG

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10746.49 Da        Isoelectric Point: 8.0168

>AT89782 WP_100955269.1 NZ_CP025113:6704464-6704763 [Bradyrhizobium sp. SK17]
MAKKLPPMHPGEVLREEFLIPLSLSAGALAKVCGVPRTRIERIANEETAISADTALRLSRALNTSAQLWLNLQNAYDVRV
AEASLANELNKIKPVVEAA

Download         Length: 300 bp

>AT89782 NZ_CP025113:6704464-6704763 [Bradyrhizobium sp. SK17]
ATGGCAAAGAAGCTGCCCCCCATGCATCCCGGCGAAGTACTTCGGGAAGAATTCCTGATCCCGTTGTCACTGTCGGCCGG
TGCGTTGGCCAAGGTCTGCGGTGTTCCGCGCACCAGGATCGAACGCATCGCAAATGAGGAAACTGCTATCTCGGCGGATA
CCGCCCTGCGCTTGTCGAGGGCGCTGAACACGTCTGCCCAGCTCTGGCTAAATCTGCAGAACGCCTATGACGTCCGCGTG
GCCGAGGCATCGCTTGCGAACGAGCTGAACAAGATCAAACCGGTGGTAGAGGCGGCCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References