Detailed information of TA system TA089751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 58482..59209
• Replicon: plasmid pSGH10
• Accession: NZ_CP025081
• Organism: Klebsiella pneumoniae strain SGH10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: SGH10_RS27440
• Protein ID: WP_011251285.1
• Coordinates: 58898..59209 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SGH10_RS27435
• Protein ID: WP_011251286.1
• Coordinates: 58482..58901 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SGH10_RS27400	53722..54120	-	399	Protein_49	hypothetical protein	-
SGH10_RS29245	54313..55068	-	756	Protein_50	hypothetical protein	-
SGH10_RS27415	55664..56107	+	444	WP_048333569.1	hypothetical protein	-
SGH10_RS27420	56128..56916	+	789	WP_040217257.1	hypothetical protein	-
SGH10_RS27425	56930..57265	+	336	Protein_53	conjugal transfer protein TraI	-
SGH10_RS27430	57367..58335	-	969	WP_074428168.1	IS5 family transposase	-
SGH10_RS27435	58482..58901	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
SGH10_RS27440	58898..59209	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
SGH10_RS27445	59414..59872	-	459	Protein_57	DDE-type integrase/transposase/recombinase	-
SGH10_RS27450	59986..60683	+	698	Protein_58	IS1 family transposase	-
SGH10_RS27455	60700..61134	+	435	Protein_59	thioredoxin fold domain-containing protein	-
SGH10_RS27460	61151..61462	+	312	WP_011251282.1	hypothetical protein	-
SGH10_RS27465	61476..61817	+	342	WP_011251281.1	hypothetical protein	-
SGH10_RS27470	61877..62833	+	957	WP_011251280.1	DsbA family protein	-
SGH10_RS27475	62950..63525	+	576	WP_074424927.1	hypothetical protein	-
SGH10_RS27480	63633..64148	+	516	WP_041937820.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..231583	231583
	-	inside	IScluster/Tn	-	-	48361..60683	12322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T89751 WP_011251285.1 NZ_CP025081:c59209-58898 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T89751 NZ_CP025081:c59209-58898 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT89751 WP_011251286.1 NZ_CP025081:c58901-58482 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT89751 NZ_CP025081:c58901-58482 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1