Detailed information of TA system TA089674

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5401968..5402563
• Replicon: chromosome
• Accession: NZ_CP025053
• Organism: Pseudomonas aeruginosa strain PB354

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: CWI22_RS25960
• Protein ID: WP_003113526.1
• Coordinates: 5402285..5402563 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CWI22_RS25955
• Protein ID: WP_003099268.1
• Coordinates: 5401968..5402273 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWI22_RS25920	5397108..5397956	+	849	WP_057382072.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
CWI22_RS25930	5398123..5399064	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
CWI22_RS25935	5399181..5399795	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
CWI22_RS25940	5399837..5400421	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
CWI22_RS25945	5400462..5401562	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
CWI22_RS25955	5401968..5402273	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
CWI22_RS25960	5402285..5402563	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CWI22_RS25970	5402892..5405120	+	2229	WP_034021308.1	TonB-dependent receptor	-
CWI22_RS25975	5405190..5405837	-	648	WP_003095021.1	carbonate dehydratase	-
CWI22_RS25980	5405899..5407137	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T89674 WP_003113526.1 NZ_CP025053:c5402563-5402285 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T89674 NZ_CP025053:c5402563-5402285 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT89674 WP_003099268.1 NZ_CP025053:c5402273-5401968 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT89674 NZ_CP025053:c5402273-5401968 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3