Detailed information of TA system TA089667

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5405131..5405726
• Replicon: chromosome
• Accession: NZ_CP025051
• Organism: Pseudomonas aeruginosa strain PB353

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: CWI23_RS26005
• Protein ID: WP_003113526.1
• Coordinates: 5405448..5405726 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CWI23_RS26000
• Protein ID: WP_003099268.1
• Coordinates: 5405131..5405436 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWI23_RS25965	5400271..5401119	+	849	WP_057382072.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
CWI23_RS25975	5401286..5402227	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
CWI23_RS25980	5402344..5402958	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
CWI23_RS25985	5403000..5403584	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
CWI23_RS25990	5403625..5404725	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
CWI23_RS26000	5405131..5405436	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
CWI23_RS26005	5405448..5405726	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CWI23_RS26015	5406055..5408283	+	2229	WP_034021308.1	TonB-dependent receptor	-
CWI23_RS26020	5408353..5409000	-	648	WP_003095021.1	carbonate dehydratase	-
CWI23_RS26025	5409062..5410300	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T89667 WP_003113526.1 NZ_CP025051:c5405726-5405448 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T89667 NZ_CP025051:c5405726-5405448 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT89667 WP_003099268.1 NZ_CP025051:c5405436-5405131 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT89667 NZ_CP025051:c5405436-5405131 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3