Detailed information of TA system TA089658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4271095..4271776
• Replicon: chromosome
• Accession: NZ_CP025050
• Organism: Pseudomonas aeruginosa strain PB368

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CWI24_RS20890
• Protein ID: WP_015503432.1
• Coordinates: 4271411..4271776 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CWI24_RS20885
• Protein ID: WP_004364117.1
• Coordinates: 4271095..4271418 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWI24_RS32560	4267015..4267173	+	159	WP_171945474.1	hypothetical protein	-
CWI24_RS32565	4267257..4267652	+	396	WP_171945473.1	hypothetical protein	-
CWI24_RS20865	4267895..4268230	+	336	WP_003140884.1	TM2 domain-containing protein	-
CWI24_RS20870	4268404..4268922	+	519	WP_003140886.1	PAAR domain-containing protein	-
CWI24_RS20875	4268919..4269620	+	702	WP_003111822.1	VRR-NUC domain-containing protein	-
CWI24_RS20880	4269637..4270725	+	1089	WP_043086021.1	DUF3396 domain-containing protein	-
CWI24_RS20885	4271095..4271418	-	324	WP_004364117.1	XRE family transcriptional regulator	Antitoxin
CWI24_RS20890	4271411..4271776	-	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CWI24_RS20895	4272069..4272308	-	240	WP_044060930.1	hypothetical protein	-
CWI24_RS20900	4272515..4272787	+	273	WP_009315277.1	hypothetical protein	-
CWI24_RS20905	4272818..4273243	-	426	WP_033991429.1	VOC family protein	-
CWI24_RS20910	4273343..4274227	+	885	WP_003140892.1	LysR family transcriptional regulator	-
CWI24_RS20915	4274200..4275153	-	954	WP_003085661.1	LysR family transcriptional regulator	-
CWI24_RS20920	4275374..4275808	+	435	WP_003158601.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4267895..4291575	23680

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-86)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T89658 WP_015503432.1 NZ_CP025050:c4271776-4271411 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T89658 NZ_CP025050:c4271776-4271411 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11896.73 Da
• Isoelectric Point: 6.4751

>AT89658 WP_004364117.1 NZ_CP025050:c4271418-4271095 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GHLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT89658 NZ_CP025050:c4271418-4271095 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
ATTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGGCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCATCTGGAAGTGATTGCACGCTTCCCGGATGGCGCGGTGAAAATCAGCAACTTCGCTGATATCGAAGAGGCGGGCGC
CTAA