Detailed information of TA system TA089653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5435615..5436210
• Replicon: chromosome
• Accession: NZ_CP025049
• Organism: Pseudomonas aeruginosa strain PB369

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: CWI25_RS26380
• Protein ID: WP_003113526.1
• Coordinates: 5435932..5436210 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CWI25_RS26375
• Protein ID: WP_003133769.1
• Coordinates: 5435615..5435920 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWI25_RS26340	5431181..5431471	-	291	WP_023083237.1	DUF5447 family protein	-
CWI25_RS26345	5431647..5431919	-	273	WP_004352675.1	hypothetical protein	-
CWI25_RS26350	5432032..5432304	+	273	WP_034021304.1	hypothetical protein	-
CWI25_RS26355	5432360..5432695	+	336	WP_025921265.1	hypothetical protein	-
CWI25_RS26360	5432856..5434232	+	1377	WP_071534372.1	DUF3696 domain-containing protein	-
CWI25_RS26365	5434229..5434711	+	483	WP_071534371.1	hypothetical protein	-
CWI25_RS26370	5434872..5435279	-	408	WP_052151012.1	hypothetical protein	-
CWI25_RS26375	5435615..5435920	-	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
CWI25_RS26380	5435932..5436210	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CWI25_RS26390	5436539..5438767	+	2229	WP_021264080.1	TonB-dependent receptor	-
CWI25_RS26395	5438838..5439485	-	648	WP_003095021.1	carbonate dehydratase	-
CWI25_RS26400	5439547..5440785	-	1239	WP_043085810.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T89653 WP_003113526.1 NZ_CP025049:c5436210-5435932 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T89653 NZ_CP025049:c5436210-5435932 [Pseudomonas aeruginosa]
CTGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAACGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT89653 WP_003133769.1 NZ_CP025049:c5435920-5435615 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT89653 NZ_CP025049:c5435920-5435615 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3