Detailed information of TA system TA089650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4238367..4239048
• Replicon: chromosome
• Accession: NZ_CP025049
• Organism: Pseudomonas aeruginosa strain PB369

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CWI25_RS20730
• Protein ID: WP_015503432.1
• Coordinates: 4238683..4239048 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CWI25_RS20725
• Protein ID: WP_004364117.1
• Coordinates: 4238367..4238690 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWI25_RS32005	4234287..4234445	+	159	WP_171945474.1	hypothetical protein	-
CWI25_RS32010	4234529..4234924	+	396	WP_171945473.1	hypothetical protein	-
CWI25_RS20705	4235167..4235502	+	336	WP_003140884.1	TM2 domain-containing protein	-
CWI25_RS20710	4235676..4236194	+	519	WP_003140886.1	PAAR domain-containing protein	-
CWI25_RS20715	4236191..4236892	+	702	WP_003111822.1	VRR-NUC domain-containing protein	-
CWI25_RS20720	4236909..4237997	+	1089	WP_043086021.1	DUF3396 domain-containing protein	-
CWI25_RS20725	4238367..4238690	-	324	WP_004364117.1	XRE family transcriptional regulator	Antitoxin
CWI25_RS20730	4238683..4239048	-	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CWI25_RS20735	4239341..4239580	-	240	WP_044060930.1	hypothetical protein	-
CWI25_RS20740	4239787..4240059	+	273	WP_009315277.1	hypothetical protein	-
CWI25_RS20745	4240090..4240515	-	426	WP_033991429.1	VOC family protein	-
CWI25_RS20750	4240615..4241499	+	885	WP_003140892.1	LysR family transcriptional regulator	-
CWI25_RS20755	4241472..4242425	-	954	WP_003085661.1	LysR family transcriptional regulator	-
CWI25_RS20760	4242646..4243080	+	435	WP_003158601.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4235167..4258847	23680

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-86)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T89650 WP_015503432.1 NZ_CP025049:c4239048-4238683 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T89650 NZ_CP025049:c4239048-4238683 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11896.73 Da
• Isoelectric Point: 6.4751

>AT89650 WP_004364117.1 NZ_CP025049:c4238690-4238367 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GHLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT89650 NZ_CP025049:c4238690-4238367 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
ATTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGGCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCATCTGGAAGTGATTGCACGCTTCCCGGATGGCGCGGTGAAAATCAGCAACTTCGCTGATATCGAAGAGGCGGGCGC
CTAA