Detailed information of TA system TA089644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3737147..3737841
• Replicon: chromosome
• Accession: NZ_CP025048
• Organism: Escherichia coli strain SC516

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: CWI33_RS18935
• Protein ID: WP_001263493.1
• Coordinates: 3737443..3737841 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: CWI33_RS18930
• Protein ID: WP_000554757.1
• Coordinates: 3737147..3737440 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWI33_RS18910	3732779..3733276	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
CWI33_RS18915	3733500..3735212	-	1713	Protein_3458	flagellar biosynthesis protein FlhA	-
CWI33_RS18920	3735184..3735969	+	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
CWI33_RS18925	3736040..3737095	+	1056	WP_001226164.1	DNA polymerase IV	-
CWI33_RS18930	3737147..3737440	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CWI33_RS18935	3737443..3737841	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CWI33_RS18940	3737851..3738303	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
CWI33_RS18945	3738549..3738752	+	204	Protein_3464	RNA ligase RtcB family protein	-
CWI33_RS18950	3738751..3739272	+	522	Protein_3465	peptide chain release factor H	-
CWI33_RS18955	3739329..3740786	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
CWI33_RS18960	3741047..3741505	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
CWI33_RS18965	3741597..3742841	+	1245	WP_000189541.1	esterase FrsA	-
-	3742101..3742181	+	81	NuclAT_11	-	-
-	3742101..3742181	+	81	NuclAT_11	-	-
-	3742101..3742181	+	81	NuclAT_11	-	-
-	3742101..3742181	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T89644 WP_001263493.1 NZ_CP025048:3737443-3737841 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T89644 NZ_CP025048:3737443-3737841 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT89644 WP_000554757.1 NZ_CP025048:3737147-3737440 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT89644 NZ_CP025048:3737147-3737440 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6