Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 788223..788725 Replicon chromosome
Accession NZ_CP025048
Organism Escherichia coli strain SC516

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CWI33_RS04140 Protein ID WP_000767829.1
Coordinates 788223..788477 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID -
Locus tag CWI33_RS04145 Protein ID WP_001610372.1
Coordinates 788474..788725 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CWI33_RS04110 783238..783465 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CWI33_RS04115 783479..784537 - 1059 WP_000492333.1 transport protein YeeE -
CWI33_RS04120 784716..786074 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CWI33_RS04125 786064..786126 - 63 WP_010723108.1 membrane protein YoeI -
CWI33_RS04130 786341..787270 - 930 WP_000803351.1 LysR family transcriptional regulator -
CWI33_RS04135 787316..788140 - 825 WP_000754737.1 SDR family oxidoreductase -
CWI33_RS04140 788223..788477 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CWI33_RS04145 788474..788725 - 252 WP_001610372.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CWI33_RS04155 789008..789058 + 51 WP_001364200.1 his operon leader peptide -
CWI33_RS04165 789204..790103 + 900 WP_000131792.1 ATP phosphoribosyltransferase -
CWI33_RS04170 790109..791413 + 1305 WP_001367667.1 histidinol dehydrogenase -
CWI33_RS04175 791410..792480 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
CWI33_RS04180 792480..793547 + 1068 WP_000080141.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T89634 WP_000767829.1 NZ_CP025048:c788477-788223 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T89634 NZ_CP025048:c788477-788223 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9289.55 Da        Isoelectric Point: 4.7954

>AT89634 WP_001610372.1 NZ_CP025048:c788725-788474 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLIDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT89634 NZ_CP025048:c788725-788474 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATAGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure

References