Detailed information of TA system TA089518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 860549..860963
• Replicon: chromosome
• Accession: NZ_CP025023
• Organism: Staphylococcus argenteus strain XNO106

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CKO49_RS04230
• Protein ID: WP_100848567.1
• Coordinates: 860549..860800 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CKO49_RS13705
• Protein ID: WP_000028424.1
• Coordinates: 860790..860963 (+)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKO49_RS04180	855890..856564	+	675	WP_088815076.1	hypothetical protein	-
CKO49_RS04185	856674..857333	-	660	WP_016028252.1	hypothetical protein	-
CKO49_RS04190	857379..858150	+	772	Protein_791	conserved phage C-terminal domain-containing protein	-
CKO49_RS04195	858160..858934	+	775	Protein_792	ATP-binding protein	-
CKO49_RS04200	858928..859086	+	159	WP_000256594.1	hypothetical protein	-
CKO49_RS04205	859099..859320	+	222	WP_001123695.1	DUF3269 family protein	-
CKO49_RS04210	859330..859735	+	406	Protein_795	DUF1064 domain-containing protein	-
CKO49_RS04215	859740..859928	+	189	WP_088808347.1	DUF3113 family protein	-
CKO49_RS04220	859929..860288	+	360	Protein_797	hypothetical protein	-
CKO49_RS04225	860292..860534	+	243	WP_100848566.1	phi PVL orf 51-like protein	-
CKO49_RS04230	860549..860800	+	252	WP_100848567.1	DUF1024 family protein	Toxin
CKO49_RS13705	860790..860963	+	174	WP_000028424.1	hypothetical protein	Antitoxin
CKO49_RS04235	860964..861244	+	281	Protein_801	hypothetical protein	-
CKO49_RS13710	861245..861406	+	162	WP_000889683.1	hypothetical protein	-
CKO49_RS04240	861421..861948	+	528	WP_072473165.1	dUTPase	-
CKO49_RS04245	861985..862191	+	207	WP_029625633.1	DUF1381 domain-containing protein	-
CKO49_RS04250	862188..862361	+	174	WP_029625632.1	transcriptional activator RinB	-
CKO49_RS04255	862362..862508	+	147	WP_000990005.1	hypothetical protein	-
CKO49_RS04260	862523..862942	+	420	Protein_807	transcriptional regulator	-
CKO49_RS04265	863128..863568	+	441	Protein_808	terminase small subunit	-
CKO49_RS04270	863555..864831	+	1277	Protein_809	PBSX family phage terminase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	845938..895889	49951

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9460.36 Da
• Isoelectric Point: 3.9373

>T89518 WP_100848567.1 NZ_CP025023:860549-860800 [Staphylococcus argenteus]
MNNREQIEQSIISASAYNGNDTEGLLKEVEDVYKKAQAFDEILDGMTNAIQHSVKEGVELDEAVRIMAGQVVYKYEEEQE
NEY

Nucleotide

• Length: 252 bp

>T89518 NZ_CP025023:860549-860800 [Staphylococcus argenteus]
ATGAATAACCGCGAACAAATTGAACAATCAATTATCAGTGCTAGTGCCTATAACGGTAATGACACAGAGGGATTACTAAA
AGAGGTTGAAGACGTGTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGATGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTGTTGAACTTGATGAAGCAGTAAGGATTATGGCAGGTCAAGTTGTCTATAAATATGAGGAGGAACAGGAA
AATGAGTATTAG

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6468.29 Da
• Isoelectric Point: 4.2484

>AT89518 WP_000028424.1 NZ_CP025023:860790-860963 [Staphylococcus argenteus]
MSISVGDKVYNHETNESLEIVQLVGDIRDTHYKLSDDSVISIIDFITKPIYLIKGDE

Nucleotide

• Length: 174 bp

>AT89518 NZ_CP025023:860790-860963 [Staphylococcus argenteus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCCGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGACGAGTGA