Detailed information of TA system TA089445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1255857..1256545
• Replicon: chromosome
• Accession: NZ_CP025000
• Organism: Idiomarina sp. X4

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: CWC33_RS06005
• Protein ID: WP_100691199.1
• Coordinates: 1256147..1256545 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: CWC33_RS06000
• Protein ID: WP_100691198.1
• Coordinates: 1255857..1256150 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWC33_RS05975	1251146..1251997	+	852	WP_100691193.1	hypothetical protein	-
CWC33_RS05980	1251992..1253095	-	1104	WP_100691194.1	DNA cytosine methyltransferase	-
CWC33_RS05985	1253096..1254853	-	1758	WP_100691195.1	Eco57I restriction-modification methylase domain-containing protein	-
CWC33_RS05995	1255362..1255685	-	324	WP_100691197.1	HigA family addiction module antidote protein	-
CWC33_RS06000	1255857..1256150	+	294	WP_100691198.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CWC33_RS06005	1256147..1256545	+	399	WP_100691199.1	type II toxin-antitoxin system YafO family toxin	Toxin
CWC33_RS06010	1256542..1257207	-	666	WP_100691200.1	hypothetical protein	-
CWC33_RS06020	1257716..1258204	+	489	WP_053953442.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15400.59 Da
• Isoelectric Point: 7.0383

>T89445 WP_100691199.1 NZ_CP025000:1256147-1256545 [Idiomarina sp. X4]
VIRVFKSKQIRQQLTDTELDALVRDFKRYKSGEGVPDFFGRDERYDHPDTFPVLRDNEVMHLHLENPDEPWPLHCIQFSK
KSNTHLVYCPAAMTSNCYLLMVILDPSAHNKAWSSSVMLPLGTMAENFRNKY

Nucleotide

• Length: 399 bp

>T89445 NZ_CP025000:1256147-1256545 [Idiomarina sp. X4]
GTGATTAGGGTTTTTAAGAGCAAACAGATTCGTCAGCAATTAACAGATACGGAACTGGATGCATTAGTTAGAGACTTTAA
ACGTTATAAAAGTGGTGAAGGCGTGCCCGACTTTTTCGGGCGCGATGAACGTTACGATCACCCCGACACTTTTCCCGTTC
TTCGTGACAATGAAGTAATGCACTTGCATCTGGAAAACCCCGATGAGCCATGGCCTTTGCATTGCATTCAATTTTCTAAG
AAAAGTAATACCCACTTGGTTTATTGCCCTGCTGCTATGACTTCTAACTGTTACTTACTCATGGTGATTTTAGACCCGTC
AGCACACAATAAAGCCTGGAGTAGTTCGGTTATGCTGCCTTTAGGAACAATGGCAGAGAACTTCAGAAATAAGTACTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10743.21 Da
• Isoelectric Point: 4.8892

>AT89445 WP_100691198.1 NZ_CP025000:1255857-1256150 [Idiomarina sp. X4]
MNTQTILAEKTVSASDVRKHPCQYFEAEPVAVLSHNKTAGYMVGAELFEAMVRQLEACNPKVESLFRPTAARLKAISADA
ERLLAEAKKSDLDDFEE

Nucleotide

• Length: 294 bp

>AT89445 NZ_CP025000:1255857-1256150 [Idiomarina sp. X4]
ATGAACACACAAACAATATTGGCTGAAAAAACAGTATCTGCCAGCGATGTACGCAAGCATCCCTGCCAGTATTTTGAAGC
TGAACCTGTGGCGGTACTCTCCCACAATAAAACCGCTGGCTATATGGTGGGTGCCGAATTATTTGAAGCTATGGTGCGCC
AGTTGGAAGCCTGTAACCCTAAAGTAGAATCCCTTTTTAGGCCGACAGCAGCGCGCCTAAAGGCTATTAGCGCCGACGCA
GAGAGGCTTTTAGCTGAAGCGAAAAAGTCTGACCTGGATGACTTTGAAGAGTGA