Detailed information of TA system TA089422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2328751..2329360
• Replicon: chromosome
• Accession: NZ_CP024996
• Organism: Herbaspirillum rubrisubalbicans strain DSM 11543

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: RC54_RS10490
• Protein ID: WP_061788830.1
• Coordinates: 2329073..2329360 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0U3KYM0
• Locus tag: RC54_RS10485
• Protein ID: WP_058895266.1
• Coordinates: 2328751..2329062 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RC54_RS10470	2324624..2325823	+	1200	WP_058895263.1	HDOD domain-containing protein	-
RC54_RS10475	2325829..2327922	+	2094	WP_061788829.1	EAL domain-containing protein	-
RC54_RS10480	2328170..2328703	+	534	WP_058895265.1	superoxide dismutase family protein	-
RC54_RS10485	2328751..2329062	-	312	WP_058895266.1	HigA family addiction module antidote protein	Antitoxin
RC54_RS10490	2329073..2329360	-	288	WP_061788830.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RC54_RS10495	2329705..2329953	-	249	WP_208652674.1	AlpA family phage regulatory protein	-
RC54_RS10500	2329950..2330840	-	891	WP_061788831.1	DUF3850 domain-containing protein	-
RC54_RS10505	2330837..2331739	-	903	WP_082803063.1	hypothetical protein	-
RC54_RS10510	2331736..2332497	-	762	WP_061788832.1	hypothetical protein	-
RC54_RS10515	2332494..2332958	-	465	WP_061788833.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2323228..2368826	45598

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (21-95)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10657.14 Da
• Isoelectric Point: 6.2085

>T89422 WP_061788830.1 NZ_CP024996:c2329360-2329073 [Herbaspirillum rubrisubalbicans]
MIISFRCAETEALFHGETVARFANIRAAAERKLQMLEAAATLDFLCSPPGNRLEPLQGDRSGQYSIRINSQFRLCFIWNS
IGEVGASHVEIVDYH

Nucleotide

• Length: 288 bp

>T89422 NZ_CP024996:c2329360-2329073 [Herbaspirillum rubrisubalbicans]
ATGATCATCAGTTTCCGATGCGCAGAAACCGAAGCCCTCTTCCATGGTGAAACCGTGGCCCGGTTCGCCAACATCCGGGC
CGCGGCAGAACGCAAACTGCAGATGCTGGAAGCCGCTGCGACGTTGGATTTCCTGTGCTCACCGCCTGGCAATCGGCTGG
AACCGCTGCAGGGTGATCGAAGCGGGCAATACAGTATCCGCATCAACAGCCAATTCCGACTCTGCTTCATCTGGAACAGC
ATTGGAGAAGTAGGCGCAAGCCATGTTGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11440.11 Da
• Isoelectric Point: 9.1470

>AT89422 WP_058895266.1 NZ_CP024996:c2329062-2328751 [Herbaspirillum rubrisubalbicans]
MNPASNRMRPIHPGEILREEFLAPANLSANALAIALRVPATRIGEIVNERRGITPDTALRLARFFGGDAQSWLNLQMTHD
LKIAQQENGARIEQEVQPMVRSA

Nucleotide

• Length: 312 bp

>AT89422 NZ_CP024996:c2329062-2328751 [Herbaspirillum rubrisubalbicans]
ATGAACCCTGCCAGCAATCGTATGCGCCCCATCCATCCGGGCGAAATCCTGCGGGAAGAATTCCTCGCCCCGGCCAACCT
CAGCGCCAATGCACTGGCCATCGCCTTGCGCGTACCCGCGACTCGCATCGGCGAAATCGTCAACGAACGGCGCGGCATCA
CGCCTGATACCGCATTGCGGCTGGCGCGATTTTTCGGTGGCGATGCGCAATCCTGGCTGAACCTGCAGATGACCCACGAC
CTCAAGATCGCCCAGCAGGAAAACGGCGCCCGCATCGAGCAGGAAGTACAACCCATGGTGCGATCGGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0U3KYM0