Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4197856..4198358 Replicon chromosome
Accession NZ_CP024978
Organism Escherichia coli strain CV839-15

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CV83915_RS24965 Protein ID WP_000767829.1
Coordinates 4198104..4198358 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CV83915_RS24960 Protein ID WP_001259255.1
Coordinates 4197856..4198107 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CV83915_RS24930 4193033..4194100 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CV83915_RS24935 4194100..4195170 - 1071 WP_063079554.1 histidinol-phosphate transaminase -
CV83915_RS24940 4195167..4196471 - 1305 WP_021571897.1 histidinol dehydrogenase -
CV83915_RS24945 4196477..4197376 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CV83915_RS24955 4197522..4197572 - 51 WP_001364200.1 his operon leader peptide -
CV83915_RS24960 4197856..4198107 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CV83915_RS24965 4198104..4198358 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CV83915_RS24970 4198441..4199265 + 825 WP_000754737.1 SDR family oxidoreductase -
CV83915_RS24975 4199311..4200240 + 930 WP_000803366.1 LysR family transcriptional regulator -
CV83915_RS24980 4200455..4200517 + 63 WP_010723108.1 membrane protein YoeI -
CV83915_RS24985 4200507..4201865 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CV83915_RS24990 4202044..4203102 + 1059 WP_000492321.1 transport protein YeeE -
CV83915_RS24995 4203116..4203343 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T89405 WP_000767829.1 NZ_CP024978:4198104-4198358 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T89405 NZ_CP024978:4198104-4198358 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT89405 WP_001259255.1 NZ_CP024978:4197856-4198107 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT89405 NZ_CP024978:4197856-4198107 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References