Detailed information of TA system TA089403

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3344664..3345391
• Replicon: chromosome
• Accession: NZ_CP024978
• Organism: Escherichia coli strain CV839-15

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: CV83915_RS20395
• Protein ID: WP_000547563.1
• Coordinates: 3344664..3344975 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CV83915_RS20400
• Protein ID: WP_000126294.1
• Coordinates: 3344972..3345391 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CV83915_RS20365	3339806..3341515	+	1710	WP_016245013.1	formate hydrogenlyase subunit HycE	-
CV83915_RS20370	3341525..3342067	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
CV83915_RS20375	3342067..3342834	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
CV83915_RS20380	3342831..3343241	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
CV83915_RS20385	3343234..3343704	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
CV83915_RS20390	3343729..3344502	+	774	WP_001026444.1	hypothetical protein	-
CV83915_RS20395	3344664..3344975	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
CV83915_RS20400	3344972..3345391	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
CV83915_RS20405	3345505..3346929	-	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
CV83915_RS20410	3346938..3348395	-	1458	WP_001107862.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
CV83915_RS20415	3348655..3349665	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
CV83915_RS20420	3349814..3350341	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T89403 WP_000547563.1 NZ_CP024978:3344664-3344975 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T89403 NZ_CP024978:3344664-3344975 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT89403 WP_000126294.1 NZ_CP024978:3344972-3345391 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT89403 NZ_CP024978:3344972-3345391 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22