Detailed information of TA system TA089390

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 35394..35766
• Replicon: chromosome
• Accession: NZ_CP024978
• Organism: Escherichia coli strain CV839-15

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A6D0I920
• Locus tag: CV83915_RS02515
• Protein ID: WP_050491434.1
• Coordinates: 35394..35588 (+)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	35587..35766 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CV83915_RS02490	31092..31313	+	222	WP_000560223.1	killing protein KilR	-
CV83915_RS02495	31313..31483	+	171	WP_001352098.1	conserved protein, Rac prophage	-
CV83915_RS02500	31558..31833	+	276	WP_000632297.1	protein RacC	-
CV83915_RS02505	31935..34535	+	2601	WP_048230494.1	exodeoxyribonuclease VIII	-
CV83915_RS02510	34528..35337	+	810	WP_000166319.1	recombination protein RecT	-
CV83915_RS02515	35394..35588	+	195	WP_050491434.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
CV83915_RS02520	35581..35791	+	211	Protein_49	double-strand break reduction protein RcbA	-
-	35587..35766	-	180	NuclAT_1	-	Antitoxin
-	35587..35766	-	180	NuclAT_1	-	Antitoxin
-	35587..35766	-	180	NuclAT_1	-	Antitoxin
-	35587..35766	-	180	NuclAT_1	-	Antitoxin
CV83915_RS02525	35870..36085	+	216	WP_000079604.1	excisionase XisR	-
CV83915_RS02530	36087..37322	+	1236	WP_000040852.1	site-specific integrase	-
CV83915_RS02535	37374..38309	+	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
CV83915_RS02540	38438..39811	-	1374	WP_016244573.1	ATP-dependent RNA helicase DbpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	582..45826	45244

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7032.91 Da
• Isoelectric Point: 8.9538

>T89390 WP_050491434.1 NZ_CP024978:35394-35588 [Escherichia coli]
MRYDNIKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T89390 NZ_CP024978:35394-35588 [Escherichia coli]
ATGAGATATGACAATATTAAACCATGTCCATTTTGTGGTTGCCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 180 bp

>AT89390 NZ_CP024978:c35766-35587 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTTACCTG
AGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTT
TCAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A6D0I920

Antitoxin