Detailed information of TA system TA089383

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 88559..88960
• Replicon: plasmid pCV839-15-p2
• Accession: NZ_CP024976
• Organism: Escherichia coli strain CV839-15

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: CV83915_RS01185
• Protein ID: WP_089565019.1
• Coordinates: 88799..88960 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	88559..88746 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CV83915_RS01155	83572..83862	-	291	WP_001299214.1	hypothetical protein	-
CV83915_RS01160	83859..85007	-	1149	WP_001024972.1	plasmid transfer ATPase TraJ	-
CV83915_RS01165	85004..85822	-	819	WP_000646097.1	IncI1-type conjugal transfer lipoprotein TraI	-
CV83915_RS01170	85941..86591	+	651	WP_000993956.1	IS66 family insertion sequence hypothetical protein	-
CV83915_RS01175	86591..86938	+	348	WP_000624618.1	IS66 family insertion sequence element accessory protein TnpB	-
CV83915_RS01180	86958..88529	+	1572	WP_000381442.1	IS66 family transposase	-
-	88559..88746	+	188	NuclAT_0	-	Antitoxin
-	88559..88746	+	188	NuclAT_0	-	Antitoxin
-	88559..88746	+	188	NuclAT_0	-	Antitoxin
-	88559..88746	+	188	NuclAT_0	-	Antitoxin
CV83915_RS01185	88799..88960	+	162	WP_089565019.1	type I toxin-antitoxin system Hok family toxin	Toxin
CV83915_RS01190	89336..89986	+	651	WP_000993956.1	IS66 family insertion sequence hypothetical protein	-
CV83915_RS01195	89986..90333	+	348	WP_000624618.1	IS66 family insertion sequence element accessory protein TnpB	-
CV83915_RS01200	90353..91924	+	1572	WP_000381442.1	IS66 family transposase	-
CV83915_RS01205	91928..92507	-	580	Protein_104	hypothetical protein	-
CV83915_RS01210	92686..93507	+	822	WP_001234485.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2	-	1..195578	195578
	-	inside	IScluster/Tn	-	-	85941..91924	5983

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6004.24 Da
• Isoelectric Point: 8.5101

>T89383 WP_089565019.1 NZ_CP024976:88799-88960 [Escherichia coli]
MKLPGNVLIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 162 bp

>T89383 NZ_CP024976:88799-88960 [Escherichia coli]
ATGAAACTACCGGGAAACGTCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTAATATTCACACTCCTGACCCG
GAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAGGTTACGGCAAGTTTGGCTTACGAATCCAGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 188 bp

>AT89383 NZ_CP024976:88559-88746 [Escherichia coli]
CTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATGAGCTATGATGTGCCCGGCGCTTGA
GGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGCAGAAAGCCCCGTAGTTAATTTTTC
ATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Antitoxin